Document Detail

Identification of tilapia ghrelin and its effects on growth hormone and prolactin release in the tilapia, Oreochromis mossambicus.
MedLine Citation:
PMID:  12831762     Owner:  NLM     Status:  MEDLINE    
We have identified ghrelin and cDNA encoding precursor protein from the stomach of a euryhaline tilapia, Oreochromis mossambicus. The sequence of 20-amino acid tilapia ghrelin is GSSFLSPSQKPQNKVKSSRI. The third serine residue was modified by n-decanoic acid. The carboxyl-terminal end of the peptide possessed an amide structure. RT-PCR analysis revealed high levels of gene expression in the stomach and low levels in the brain, kidney and gill. Tilapia ghrelin stimulated growth hormone (GH) and prolactin (PRL) release from the organ-cultured tilapia pituitary at a dose of 10 nM. Thus, a novel regulatory mechanism of GH secretion by gastric ghrelin seems to be conserved in the tilapia. Stimulation of PRL release by homologous ghrelin has been reported in human, bullfrog and eel, and suggests the presence of growth hormone secretagogue receptor not only on somatotrophs but also on PRL cells of the tilapia pituitary.
Hiroyuki Kaiya; Masayasu Kojima; Hiroshi Hosoda; Larry G Riley; Tetsuya Hirano; E Gordon Grau; Kenji Kangawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  135     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-30     Completed Date:  2004-03-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  421-9     Citation Subset:  IM    
Department of Biochemistry, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Osaka 565-8565, Suita, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Gene Expression Profiling
Growth Hormone / metabolism,  secretion*
Molecular Sequence Data
Organ Specificity
Peptide Hormones / chemistry,  genetics,  isolation & purification,  metabolism*
Pituitary Gland / chemistry,  cytology,  secretion
Prolactin / metabolism,  secretion*
RNA, Messenger / genetics,  metabolism
Sequence Homology
Stomach / chemistry
Tilapia / metabolism*
Reg. No./Substance:
0/Ghrelin; 0/Peptide Hormones; 0/RNA, Messenger; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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