| Identification of tilapia ghrelin and its effects on growth hormone and prolactin release in the tilapia, Oreochromis mossambicus. | |
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MedLine Citation:
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PMID: 12831762 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have identified ghrelin and cDNA encoding precursor protein from the stomach of a euryhaline tilapia, Oreochromis mossambicus. The sequence of 20-amino acid tilapia ghrelin is GSSFLSPSQKPQNKVKSSRI. The third serine residue was modified by n-decanoic acid. The carboxyl-terminal end of the peptide possessed an amide structure. RT-PCR analysis revealed high levels of gene expression in the stomach and low levels in the brain, kidney and gill. Tilapia ghrelin stimulated growth hormone (GH) and prolactin (PRL) release from the organ-cultured tilapia pituitary at a dose of 10 nM. Thus, a novel regulatory mechanism of GH secretion by gastric ghrelin seems to be conserved in the tilapia. Stimulation of PRL release by homologous ghrelin has been reported in human, bullfrog and eel, and suggests the presence of growth hormone secretagogue receptor not only on somatotrophs but also on PRL cells of the tilapia pituitary. |
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Authors:
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Hiroyuki Kaiya; Masayasu Kojima; Hiroshi Hosoda; Larry G Riley; Tetsuya Hirano; E Gordon Grau; Kenji Kangawa |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology Volume: 135 ISSN: 1096-4959 ISO Abbreviation: Comp. Biochem. Physiol. B, Biochem. Mol. Biol. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-06-30 Completed Date: 2004-03-17 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9516061 Medline TA: Comp Biochem Physiol B Biochem Mol Biol Country: England |
Other Details:
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Languages: eng Pagination: 421-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Osaka 565-8565, Suita, Japan. kaiya@ri.ncvc.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Gene Expression Profiling Ghrelin Growth Hormone / metabolism, secretion* Humans Molecular Sequence Data Organ Specificity Peptide Hormones / chemistry, genetics, isolation & purification, metabolism* Pituitary Gland / chemistry, cytology, secretion Prolactin / metabolism, secretion* RNA, Messenger / genetics, metabolism Sequence Homology Stomach / chemistry Tilapia / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Ghrelin; 0/Peptide Hormones; 0/RNA, Messenger; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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