| Identification of three ABC transporter genes in Trypanosoma brucei spp. | |
| | |
MedLine Citation:
|
PMID: 9493208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
ABC transporters are key players in the multidrug resistance of cancer cells and yeast, and they appear to be involved in the drug resistance of various pathogenic protozoa. No member of this ubiquitous protein family has yet been described in Trypanosoma brucei spp., the causative agents of African sleeping sickness and animal trypanosomiases. However, different cases of artificially induced drug resistance were shown to be linked to a reduction in net drug uptake. We used polymerase chain reaction with degenerate oligonucleotide primers corresponding to particularly conserved regions within the ATP-binding cassette to probe the genome of T. brucei spp. for the presence of ABC transporter genes. Three different sequence segments encoding ATP-binding cassettes were identified, which, upon Southern blotting, appeared to belong to distinct genes designated Tbabc1, Tbabc2, and Tbabc3. They appear to be single-copy genes in both drug-susceptible and drug-resistant stocks of T. brucei spp., expressed in bloodstream forms as well as in the procyclic life stage. Whereas Tbabc3 shows moderate homology to various known ABC transporters, Tbabc1 and Tbabc2 are highly homologous to P-glycoprotein A of Leishmania tarentolae and to the multidrug resistance protein 1 of L. donovani, respectively. |
| | |
Authors:
|
P Mäser; R Kaminsky |
Related Documents
:
|
21615008 - Drugs, the internet and change. 10332068 - How interactions between drugs and agarose-carrageenan hydrogels influence the simultan... 11510488 - Transport characteristics of ebastine and its metabolites across human intestinal epith... 18624678 - The pregnane x receptor: from bench to bedside. 19002788 - Vault-related resistance to anticancer drugs determined by the expression of the major ... 11270718 - Inhibitors of bacterial topoisomerases: mechanisms of action and resistance and clinica... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Parasitology research Volume: 84 ISSN: 0932-0113 ISO Abbreviation: Parasitol. Res. Publication Date: 1998 |
Date Detail:
|
Created Date: 1998-04-13 Completed Date: 1998-04-13 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8703571 Medline TA: Parasitol Res Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 106-11 Citation Subset: IM |
Affiliation:
|
Swiss Tropical Institute, Basel, Switzerland. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/U89026; U89027; U89028 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
ATP-Binding Cassette Transporters
/
chemistry,
genetics*,
isolation & purification Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular Gene Expression Regulation, Developmental Genes, Protozoan / genetics Molecular Sequence Data Polymerase Chain Reaction RNA-Directed DNA Polymerase Sequence Analysis, DNA Sequence Homology, Amino Acid Trypanosoma brucei brucei / chemistry, genetics*, growth & development |
| Chemical | |
Reg. No./Substance:
|
0/ATP-Binding Cassette Transporters; EC 2.7.7.49/RNA-Directed DNA Polymerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of female gonadal hormones on the parasitemia of female Calomys callosus infected with the...
Next Document: Genetic diversity in porcine Oesophagostomum dentatum and O. quadrispinulatum and their delineation ...