| Identification of a surface epitope of human erythropoietin with anti-peptide antibodies. | |
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MedLine Citation:
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PMID: 2409968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antibodies reactive with human erythropoietin were isolated from the serum of rabbits immunized with a twenty-six amino acid synthetic polypeptide corresponding to a proposed NH2-terminal sequence of the hormone. As shown by inhibition with peptide fragments, those antibodies that bound to erythropoietin recognized the (8-15) domain, strongly suggesting tht this region is exposed on the hormone's surface. This was confirmed by affinity purification of these antibodies on immobilized fragment (8-15). These results provide insight into the tertiary structure of human erythropoietin and suggest uses for the sequence-specific antibodies in labeling the hormone. |
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Authors:
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A J Sytkowski; D M Wojchowski |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 129 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1985 Jun |
Date Detail:
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Created Date: 1985-08-09 Completed Date: 1985-08-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 707-13 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Binding Sites Epitopes / analysis* Erythropoietin / analysis, immunology* Humans Kinetics Peptide Fragments / immunology* Protein Conformation |
| Grant Support | |
ID/Acronym/Agency:
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AM 01849/AM/NIADDK NIH HHS; AM 34041/AM/NIADDK NIH HHS; HL 32262/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Epitopes; 0/Peptide Fragments; 11096-26-7/Erythropoietin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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