Document Detail


Identification of subpopulations of rat granulosa cells: sedimentation properties and hormonal responsiveness.
MedLine Citation:
PMID:  2990865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ovarian granulosa cells from small follicles have generally been considered to comprise a homogeneous cell population; however, stratified arrangements of hormone receptors have been found in antral and mural granulosa cells of Graafian follicles. Using cultured granulosa cells derived from immature, hypophysectomized, estrogen-treated rats, we have previously shown that vasoactive intestinal peptide (VIP) as well as FSH stimulates steroid production by these cells. Dose-response analysis indicated that the actions of these hormones were additive, suggesting the presence of subpopulations of granulosa cells. Using a continuous (0-30%) Metrizamide density gradient, we have identified three populations of granulosa cells with different sedimentation properties. After centrifugation for 15 min at 1500 X g, cells sedimented at Metrizamide concentrations of 13%, 18%, and 20% (peaks A, B, and C, respectively). The subpopulation with lowest density (peak A) comprised 5% of the total cells, whereas the remaining cells were distributed approximately equally in the other two peaks. The profiles of estrogen and progesterone production by these cells in response to FSH and VIP indicated that FSH preferentially stimulated steroid production in cells with the highest density (peak C), whereas VIP mainly induced steroidogenic responses in cells of intermediate density (peak B). In contrast, cells with the lowest density (peak A) were unresponsive to either hormone. Treatment with forskolin, a universal adenylate cyclase activator, induced steroid production in both subpopulations B and C. Further studies demonstrated that LH/human CG receptors were induced by FSH and forskolin in cells from peak C, whereas VIP treatment did not induce LH/human CG receptors in cells from peak B. In unfractionated cultured cells, GnRH potently antagonized FSH- but not VIP-induced steroidogenesis. Upon density-gradient fractionation, the profile of GnRH receptor content correlated well with GnRH effects since FSH-responsive cells (peak C) contained the majority of GnRH receptors. The present results demonstrate that granulosa cells from immature follicles are heterogeneous and consist of two major subpopulations of cells with differential responsiveness to FSH and VIP. These findings provide the basis for further morphological and biochemical analysis of subpopulations of granulosa cells during follicular development.
Authors:
B G Kasson; R Meidan; J B Davoren; A J Hsueh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  117     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-09-19     Completed Date:  1985-09-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1027-34     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Centrifugation, Density Gradient
Estrogens / biosynthesis
Female
Follicle Stimulating Hormone / pharmacology*
Gonadotropin-Releasing Hormone / analogs & derivatives,  pharmacology
Granulosa Cells / cytology*,  drug effects
Progesterone / biosynthesis
Rats
Receptors, Cell Surface / biosynthesis,  metabolism
Receptors, LH
Receptors, LHRH
Vasoactive Intestinal Peptide / pharmacology*
Grant Support
ID/Acronym/Agency:
HD-06502/HD/NICHD NIH HHS; HD-12303/HD/NICHD NIH HHS; HD-14084/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Estrogens; 0/Receptors, Cell Surface; 0/Receptors, LH; 0/Receptors, LHRH; 33515-09-2/Gonadotropin-Releasing Hormone; 37221-79-7/Vasoactive Intestinal Peptide; 52435-06-0/surfagon; 57-83-0/Progesterone; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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