Document Detail


Identification and structural analysis of synthetic oligosaccharides of Shigella sonnei using MALDI-TOF MS.
MedLine Citation:
PMID:  11527533     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MALDI-TOF mass spectroscopy was used for the molecular weight determination of protected synthetic oligosaccharides related to a cell surface bacterial polysaccharide. By-products containing chlorinated protecting groups caused isotopic patterns characteristic of the natural isotopic distribution of chlorine, were identified on the basis of isotopic distribution. 2,4,6-Trihydroxyacetophenone (THAP) as a matrix was better than 2,5-dihydroxybenzoic acid (DHB) for compounds containing chlorine, since monoisotopic resolution and no fragmentation were observed. In the post source decay (PSD) mode the identification of the oligosaccharide sequence through cleavage of the interglycosidic linkages was also possible, thus providing a sensitive and accurate tool for the structural verification of synthetic oligosaccharide intermediates.
Authors:
G Gyémánt; A Tóth; I Bajza; L Kandra; A Lipták
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carbohydrate research     Volume:  334     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-08-30     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  315-22     Citation Subset:  IM    
Affiliation:
Faculty of Sciences, Institute of Biochemistry, University of Debrecen, H-4010, PO Box 55, Debrecen, Hungary. gyemant@tigris.klte.hu
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Conformation
Carbohydrate Sequence
Molecular Sequence Data
Oligosaccharides / chemical synthesis,  chemistry*
Shigella sonnei / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Chemical
Reg. No./Substance:
0/Oligosaccharides

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