| Identification and structural analysis of synthetic oligosaccharides of Shigella sonnei using MALDI-TOF MS. | |
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MedLine Citation:
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PMID: 11527533 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MALDI-TOF mass spectroscopy was used for the molecular weight determination of protected synthetic oligosaccharides related to a cell surface bacterial polysaccharide. By-products containing chlorinated protecting groups caused isotopic patterns characteristic of the natural isotopic distribution of chlorine, were identified on the basis of isotopic distribution. 2,4,6-Trihydroxyacetophenone (THAP) as a matrix was better than 2,5-dihydroxybenzoic acid (DHB) for compounds containing chlorine, since monoisotopic resolution and no fragmentation were observed. In the post source decay (PSD) mode the identification of the oligosaccharide sequence through cleavage of the interglycosidic linkages was also possible, thus providing a sensitive and accurate tool for the structural verification of synthetic oligosaccharide intermediates. |
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Authors:
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G Gyémánt; A Tóth; I Bajza; L Kandra; A Lipták |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carbohydrate research Volume: 334 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-08-30 Completed Date: 2001-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 315-22 Citation Subset: IM |
Affiliation:
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Faculty of Sciences, Institute of Biochemistry, University of Debrecen, H-4010, PO Box 55, Debrecen, Hungary. gyemant@tigris.klte.hu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Conformation Carbohydrate Sequence Molecular Sequence Data Oligosaccharides / chemical synthesis, chemistry* Shigella sonnei / chemistry* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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