Document Detail


Identification, structural analysis and function of hyaluronan in developing fish larvae (leptocephali).
MedLine Citation:
PMID:  12031471     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hyaluronan (HA) has been identified as the principal glycosaminoglycan (CAG) in the highly hydrated, extracellular body matrix of the larval stage (leptocephalus) of seven species of true eels (Teleostei: Elopomorpha: Anguilliformes) and the ladyfish Elops saurus (Elopiformes), and was found as a minor GAG component in the bonefish Albula sp. (Albuliformes). Identification was based on: (1) HPLC separation of unsaturated disaccharides derived from chondroitinase ABC digests of whole-body GAG extracts; (2) 1H NMR analyses of native GAG polymers; and (3) degradation of GAG extracts by Streptomyces hyaluronan lyase. The unsaturated disaccharide 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-glucose (DeltaDi-HA) accounted for 92.4-99.8% of the total disaccharides in chondroitinase digests. Trace amounts of unsaturated disaccharides of chondroitin sulfate were also present. Two-dimensional gCOSY spectra of the native HA polymer were similar for all species. Proton assignments for the HA disaccharide repeat (GlcAbeta1-3GlcNAcbeta1-4) in D(2)O, based on gCOSY, DQF-COSY and TOCSY analyses for the eel Ahlia egmontis, were concordant with published chemical shifts for HA oligosaccharides. In addition to its presumed role in maintaining the structural integrity and hydration of the gelatinous body of the leptocephalus, HA is postulated to function as a storage polysaccharide in those species in which it is the predominant GAG.
Authors:
Edward Pfeiler; Hidenao Toyoda; Michael D Williams; Ronald A Nieman
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  132     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-28     Completed Date:  2003-01-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  443-51     Citation Subset:  IM    
Affiliation:
Centro de Investigación en Alimentación y Desarrollo, A.C., Unidad Guaymas, Apartado Postal 284, Guaymas, Sonora 85480, Mexico. epfeiler@asu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Chondroitin ABC Lyase / metabolism
Chromatography, High Pressure Liquid
Disaccharides / analysis,  chemistry
Eels / growth & development,  metabolism
Fishes / growth & development*,  metabolism
Hyaluronic Acid / analysis,  chemistry*,  metabolism*
Larva / chemistry*,  growth & development*
Magnetic Resonance Spectroscopy
Polysaccharide-Lyases / metabolism
Streptomyces / enzymology
Chemical
Reg. No./Substance:
0/Disaccharides; 9004-61-9/Hyaluronic Acid; EC 4.2.2.-/Polysaccharide-Lyases; EC 4.2.2.1/hyaluronate lyase; EC 4.2.2.20/Chondroitin ABC Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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