Document Detail


Identification of sperm forward motility-related proteins in human seminal plasma.
MedLine Citation:
PMID:  17393427     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Seminal plasma, an amorphous material that exists in semen, contains proteins related to sperm forward motility. Employing affinity chromatography with ConA beads and protein ultrafiltration, we isolated and concentrated proteins from heated human seminal plasma. Results of computer-assisted semen analyses (CASA) demonstrated that the forward motility index of bovine spermatozoa from the epididymal caput, incubated with proteins and theophylline, was significantly different from that of spermatozoa incubated with theophylline alone (P < 0.01). The electrophoreses revealed that the protein bands with high molecular weights in the gel of PAGE changed into low molecular weights in the gel of SDS-PAGE. Furthermore, proteins from a separated portion of the PAGE gel were still able to stimulate spermatozoa from the epididymal caput to gain forward motility. Two-dimensional (2D)-gel electrophoresis and mass spectrometry indicated that spots focused on the portion seemed, according to their amino acid sequences, to be like human alpha-1-antitrypsin and zinc-alpha-2-glycoprotein (ZAG) precursors. Western blot analysis showed the presence of these two proteins in seminal plasma. These proteins, related to the forward motility of spermatozoa in human seminal plasma, may play important roles during maturation of spermatozoa, from the epididymis through fertilization in the female reproductive tract.
Authors:
Zhide Ding; Fei Qu; Wei Guo; Xiaoqian Ying; Mingzhang Wu; Yonglian Zhang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  74     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-07-03     Completed Date:  2007-11-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1124-31     Citation Subset:  IM    
Affiliation:
Department of Histology and Embryology, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory for Reproductive Medicine, Shanghai, China. zding@shsmu.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Blotting, Western
Carrier Proteins / chemistry,  isolation & purification*
Cattle
Chemical Fractionation
Chromatography, High Pressure Liquid
Electrophoresis, Gel, Two-Dimensional
Glycoproteins / chemistry,  isolation & purification*
Humans
Male
Molecular Weight
Semen / chemistry*
Sperm Motility*
alpha 1-Antitrypsin / chemistry,  isolation & purification*
Chemical
Reg. No./Substance:
0/AZGP1 protein, human; 0/Carrier Proteins; 0/Glycoproteins; 0/alpha 1-Antitrypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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