| Identification of small molecule inhibitors of telomerase activity through transcriptional regulation of hTERT and calcium induction pathway in human lung adenocarcinoma A549 cells. | |
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MedLine Citation:
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PMID: 20813535 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High telomerase activity (TA) is detected in most cancer cells; and thus, TA inhibition by drug or dietary food components is a new strategy for cancer prevention. In this report, we examined the effects of fourteen natural or synthetic compounds on TA in human lung adenocarcinoma A549 cells. The results demonstrated that some of the tested compounds inhibited TA, being 2'-hydroxy-2,3,4',6'-tetramethoxychalcone (HTMC) was the most potent among tested. In A549 cells, HTMC also inhibited the cell proliferation, decreased the expression of human telomerase reverse transcriptase (hTERT) and sequentially reduced the hTERT promoter. In soft agar assay HTMC treatment reduced the colony formation of A549 cells. Cellular fractionation and immunofluorescence assay demonstrated that there was no translocation of hTERT from nuclei to cytoplasm. Further studies revealed that the release of Ca(2+) was the underlying mechanism of suppressed TA and hTERT transcription in A549 cells exposed to HTMC. These in vitro data support the development of HTMC as a therapeutic agent for cancer complications. |
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Authors:
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Yerra Koteswara Rao; Te-Yu Kao; Ming-Fang Wu; Jiunn-Liang Ko; Yew-Min Tzeng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-13 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry Volume: 18 ISSN: 1464-3391 ISO Abbreviation: Bioorg. Med. Chem. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-20 Completed Date: 2011-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9413298 Medline TA: Bioorg Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 6987-94 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Wufeng, Taiwan, ROC. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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enzymology,
metabolism* Antineoplastic Agents / chemical synthesis, chemistry, pharmacology* Calcium / metabolism* Cell Line, Tumor Cell Proliferation / drug effects Dose-Response Relationship, Drug Drug Screening Assays, Antitumor Enzyme Inhibitors / chemical synthesis, chemistry, pharmacology* Humans Lung Neoplasms / enzymology, metabolism* Molecular Structure Molecular Weight Promoter Regions, Genetic / drug effects, genetics Structure-Activity Relationship Telomerase / antagonists & inhibitors*, genetics, metabolism Transcription, Genetic / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Enzyme Inhibitors; 7440-70-2/Calcium; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase |
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