| Identification of a single sinusoidal bile salt uptake system in skate liver. | |
MedLine Citation:
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PMID: 3425718 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To identify the sinusoidal bile acid uptake system(s) of skate liver, photoaffinity labeling and kinetic transport studies were performed in isolated plasma membranes as well as intact hepatocytes. In both preparations photoaffinity labeling with the photolabile bile salt derivative (7,7-azo-3 alpha, 12 alpha-dihydroxy-5 beta-[3 beta-3H]cholan-24-oyl)-2-aminoethanesulfonate revealed the presence of a predominant bile salt binding polypeptide with an apparent molecular weight of 54,000. The labeling of this polypeptide was inhibited by taurocholate and cholate in a concentration-dependent manner and was virtually abolished by 1 mM of the anion transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. Kinetic studies of hepatic uptake with taurocholate, cholate, and the photoreactive bile salt derivative indicated the involvement of a single transport system, and all three substrates mutually competed with the uptake of each other. Finally, irreversible inhibition of the bile salt uptake system by photoaffinity labeling of hepatocytes with high concentrations (250 microM) of photolabile derivative reduced the Vmax but not the Km of taurocholate uptake. These findings strongly indicate that a single polypeptide with an apparent molecular weight of 54,000 is involved in sinusoidal bile salt uptake into skate hepatocytes. These findings contrast with similar studies in rat liver that implicate both a 54,000- and 48,000-K polypeptide in bile salt uptake and are consistent with a single Na+-independent transport mechanism for hepatic bile salt uptake in this primitive vertebrate. |
Authors:
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G Fricker; G Hugentobler; P J Meier; G Kurz; J L Boyer |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 253 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1987 Dec |
Date Detail:
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Created Date: 1988-01-27 Completed Date: 1988-01-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G816-22 Citation Subset: IM |
Affiliation:
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Mount Desert Island Biological Laboratory, Salsbury Cove, Maine 04672. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Affinity Labels Animals Anions Bile Acids and Salts / metabolism* Biological Transport Cell Membrane / metabolism Cholic Acid Cholic Acids / metabolism Electric Fish / physiology* Liver / metabolism* Membrane Proteins / metabolism Molecular Weight Skates (Fish) / physiology* Taurocholic Acid / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DK-25636/DK/NIDDK NIH HHS; DK-34989/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Affinity Labels; 0/Anions; 0/Bile Acids and Salts; 0/Cholic Acids; 0/Membrane Proteins; 81-24-3/Taurocholic Acid; 81-25-4/Cholic Acid |
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