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Identification of seed dormancy mutants by activation tagging.
MedLine Citation:
PMID:  21898257     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Activation tagging is an important tool for gene discovery in plants. This method utilizes a T-DNA sequence that contains four tandem copies of the cauliflower mosaic virus 35S enhancer sequence or promoters oriented outward to the T-DNA border sequences. These elements enhance the expression of genes neighboring on either side of the randomly integrated T-DNA, resulting in gain-of-function phenotypes. Activation tagging has identified a number of genes, including those fundamental to plant development, such as the floral inducer gene, FLOWERING LOCUS T  (FT  ). The methods surrounding activation-tagging approaches are described in this chapter. While seeds have generally not been the targets of these methods in the past, activation tagging provides a powerful approach to uncover genes involved in seed dormancy and germination, including those that mediate hormone signal transduction.
Authors:
Tiehan Zhao; Ying Zeng; Allison R Kermode
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  773     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-09-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  185-98     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.
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