Document Detail


Identification of a retinoic acid-responsive neural enhancer in the Ciona intestinalis Hox1 gene.
MedLine Citation:
PMID:  23302037     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The Hox1 gene in the urochordate ascidian Ciona intestinalis (Ci-Hox1) is expressed in the nerve cord and epidermis. We identified a nerve cord enhancer in the second intron of Ci-Hox1, and demonstrated that retinoic acid (RA) plays a major role in activating this enhancer. The enhancer contained a putative retinoic acid-response element (RARE). Mutation of the RARE in the Ci-Hox1 nerve cord enhancer only partially abolished the enhancer activity. Genes encoding RA synthase and the RA receptor were knocked down using specific antisense morpholino oligos (MOs), and injection of embryos with these MOs resulted in the complete disappearance of epidermal expression of Ci-Hox1 and reduction of neural expression. However, nerve cord expression was not completely repressed. These results suggest that the nerve cord enhancer is activated by two partially redundant pathways; one RA-dependent and one RA-independent.
Authors:
Miyuki Kanda; Taku Ikeda; Shigeki Fujiwara
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Development, growth & differentiation     Volume:  -     ISSN:  1440-169X     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors Development, Growth & Differentiation © 2013 Japanese Society of Developmental Biologists.
Affiliation:
Department of Applied Science, Kochi University, 2-5-1 Akebono-cho, Kochi, 780-8520, Japan.
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