Document Detail

Identification of a restriction point at the M/G1 transition in CHO cells.
MedLine Citation:
PMID:  15004698     Owner:  NLM     Status:  MEDLINE    
The regulation of cell cycle progression in normal mammalian cells is dependent on the presence of growth factors. In their absence, non-transformed cells will stop dividing and enter the quiescent state (G0). We show here that in Chinese hamster ovary cells, at least two serum-dependent points exist during G1 that lead to different cellular responses. The first point is located immediately after mitosis and is suggested to link with apoptosis. The second point is located late in G1, and probably corresponds with the 'classic' restriction point R. Cells depleted of serum after the first restriction point will not stop randomly in G1 but continue G1 progression until they reach the late restriction point, as marked by translocation of p42(MAPkinase) (ERK2) to the nucleus.
E Hullemann; J J M Bijvelt; A J Verkleij; C T Verrips; J Boonstra
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  61     ISSN:  1420-682X     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-08     Completed Date:  2004-04-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  600-9     Citation Subset:  IM    
Department of Molecular Cell Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
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MeSH Terms
Apoptosis / physiology
CHO Cells
G1 Phase / physiology*
Mitogen-Activated Protein Kinases / metabolism
Mitosis / physiology*
Serum / physiology
Reg. No./Substance:
EC Protein Kinases

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