| Identification of regions in alleles of the flax rust resistance gene L that determine differences in gene-for-gene specificity. | |
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MedLine Citation:
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PMID: 10072407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thirteen alleles (L, L1 to L11, and LH) from the flax L locus, which encode Toll/interleukin-1 receptor homology-nucleotide binding site-leucine-rich repeat (TIR-NBS-LRR) rust resistance proteins, were sequenced and compared to provide insight into their evolution and into the determinants of gene-for-gene resistance specificity. The predicted L6 and L11 proteins differ solely in the LRR region, whereas L6 and L7 differ solely in the TIR region. Thus, specificity differences between alleles can be determined by both the LRR and TIR regions. Functional analysis in transgenic plants of recombinant alleles constructed in vitro provided further information: L10-L2 and L6-L2 recombinants, encoding the LRR of L2, conferred L2 resistance specificity, and an L2-L10 recombinant, encoding the LRR of L10, conferred a novel specificity. The sequence comparisons also indicate that the evolution of L alleles has probably involved reassortment of variation, resulting from accumulated point mutations, by intragenic recombination. In addition, large deletion events have occurred in the LRR-encoding regions of L1 and L8, and duplication events have occurred in the LRR-encoding region of L2. |
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Authors:
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J G Ellis; G J Lawrence; J E Luck; P N Dodds |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Plant cell Volume: 11 ISSN: 1040-4651 ISO Abbreviation: Plant Cell Publication Date: 1999 Mar |
Date Detail:
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Created Date: 1999-05-18 Completed Date: 1999-05-18 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 9208688 Medline TA: Plant Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 495-506 Citation Subset: IM |
Affiliation:
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Commonwealth Scientific and Industrial Research Organization-Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia. jef.ellis@pi.csiro.au |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF093638; AF093639; AF093640; AF093641; AF093642; AF093643; AF093644; AF093645; AF093646; AF093647; AF093648; AF093649 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles* Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Plant / chemistry Flax / genetics* Genetic Variation* Immunity, Innate / genetics Molecular Sequence Data Plant Diseases / genetics* Receptors, Interleukin-1 / genetics* Repetitive Sequences, Nucleic Acid Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/DNA, Plant; 0/Receptors, Interleukin-1 |
| Comments/Corrections | |
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