Document Detail


Identification of region specific genes by chromosome microdissection.
MedLine Citation:
PMID:  9062577     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosome microdissection is an extremely useful molecular cytogenetic tool for the characterization of chromosomal abnormalities in tumor cells. Although it has been used primarily in conjunction with fluorescence in situ hybridization (FISH), microdissection has also been useful for molecular analysis via microclone library construction. Recently, microdissection has been applied to the isolation of region specific cDNAs with sufficient success that gene discovery can now be added to the list of applications of this versatile molecular cytogenetic technique.
Authors:
P S Meltzer; X Y Guan; Y A Su; E Gracia; J M Trent
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  93     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-26     Completed Date:  1997-03-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  29-32     Citation Subset:  IM    
Affiliation:
Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda, Maryland 20892-4470, USA.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Mapping / methods*
DNA, Complementary / isolation & purification
Gene Amplification
Humans
In Situ Hybridization, Fluorescence / methods*
Polymerase Chain Reaction / methods
Chemical
Reg. No./Substance:
0/DNA, Complementary

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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