Document Detail


Identification and radiation hybrid mapping of members of the porcine proteasome/ubiquitin system.
MedLine Citation:
PMID:  10721427     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the identification and radiation hybrid mapping of members of the proteasome/ubiquitin system in pigs that, so far, have only been identified in humans and cattle. Expressed sequence tags (ESTs) were constructed from ten oligo(dT)-primed individually tagged, directionally cloned and normalized cDNA libraries from peripheral blood cells (PBC), spleen (Sp), thymus (Th), lymph node (LN) and bone marrow (BM) from immunologically naive and challenged pigs as part of an implant-associated orthopedic infection model. The ESTs mapped using the 7000 rad IMpRH panel (Hawken et al., 1999) were ubiquitin fusion-degradation 1 like protein (UFD1L), ubiquitin activating enzyme E1 and ubiquitin-S27a fusion protein which mapped to porcine chromosomes 14, 7 and X, respectively.
Authors:
A Rink; E M Santschi; K M Mendoza; L J Alexander; C W Beattie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Animal biotechnology     Volume:  10     ISSN:  1049-5398     ISO Abbreviation:  Anim. Biotechnol.     Publication Date:  1999  
Date Detail:
Created Date:  2000-04-19     Completed Date:  2000-04-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9011409     Medline TA:  Anim Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  133-5     Citation Subset:  IM    
Affiliation:
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Mapping*
Cysteine Endopeptidases / genetics*
DNA, Complementary / genetics
Multienzyme Complexes / genetics*
Proteasome Endopeptidase Complex
Protein Multimerization
Recombinant Fusion Proteins / genetics
Swine / genetics*
Ubiquitins / genetics*
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Multienzyme Complexes; 0/Recombinant Fusion Proteins; 0/Ubiquitins; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.25.1/Proteasome Endopeptidase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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