| Identification and quantitative determination of a major circulating metabolite of gambogic acid in human. | |
| | |
MedLine Citation:
|
PMID: 20149761 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Gambogic acid (GA), a promising anticancer candidate, is a polyprenylated xanthone abundant in the resin of Garcinia morella and Garcinia hanburyi. The major circulating metabolite of GA in human, 10-hydroxygambogic acid (10-OHGA), was identified by comparison of the retention time and mass spectra with those of reference standard using liquid chromatography-tandem mass spectrometry. The reference standard of 10-OHGA was isolated from bile samples of rats after intravenous injection of GA injection, and its structure was confirmed by NMR. Then, a selective and sensitive method was developed for the quantitative determination of this metabolite in human plasma. After liquid-liquid extraction by ethyl acetate, the analyte and the internal standard were separated on a Sepax HPC18 column (100 mm x 2.1 mm i.d., 3.0 microm) with a mobile phase of 10mM ammonium acetate water solution containing 0.1% formic acid-acetonitrile (20:80, v/v). The detection was performed on a single quadrupole mass spectrometer equipped with electrospray ionization (ESI) source. The calibration curve was linear over the range of 3-2000 ng/mL for 10-OHGA. The developed quantification method can now be used for the pharmacokinetic and pharmacological studies of 10-OHGA after intravenous infusion of GA injection in human. |
| | |
Authors:
|
Jing Yang; Li Ding; Shaohong Jin; Xiaoxue Liu; Wenyuan Liu; Zhenzhong Wang |
Publication Detail:
|
Type: Journal Article Date: 2010-01-29 |
Journal Detail:
|
Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 878 ISSN: 1873-376X ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-02-22 Completed Date: 2010-06-15 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 659-66 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analysis of Variance Animals Bile / chemistry*, metabolism Chromatography, Liquid / methods* Drug Stability Humans Rats Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization / methods* Xanthones / blood*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/10-hydroxygambogic acid; 0/Xanthones; 2752-65-0/gambogic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Investigation of geometric distortions on magnetic resonance and cone beam computed tomography image...
Next Document: Simultaneous determination of glucuronic acid and sulfuric acid conjugated metabolites of daidzein a...