Document Detail

Identification and quantitation of all-trans- and 13-cis-retinoic acid and 13-cis-4-oxoretinoic acid in human plasma.
MedLine Citation:
PMID:  2280185     Owner:  NLM     Status:  MEDLINE    
Human plasma was analyzed by high performance liquid chromatography for the presence of retinoic acid and 4-oxoretinoic acid isomers. Peaks that coeluted with the reference compounds all-trans-retinoic acid, 13-cis-retinoic acid, and 13-cis-4-oxoretinoic acid were routinely observed in human plasma. These retinoids were unequivocally identified by the following methods: comigration with reference compounds under several high performance liquid chromatographic conditions; comparison of ultraviolet spectra with those of reference compounds; derivatization with diazomethane and coelution of the methyl esters with reference compounds in a high performance liquid chromatographic system as well as in a gas chromatography system with a mass selective detector. In vitro formation of 13-cis-retinoic acid and 13-cis-4-oxoretinoic acid as artifacts during the analytical procedure was excluded by control experiments. The mean plasma concentrations of the vitamin A metabolites in ten male volunteers were: all-trans-retinoic acid: 1.32 +/- 0.46 ng/ml; 13-cis-retinoic acid: 1.63 +/- 0.85 ng/ml; and 13-cis-4-oxoretinoic acid: 3.68 +/- 0.99 ng/ml. After oral dosing with vitamin A (833 IU/kg body weight) in five male volunteers, mean plasma all-trans-retinoic acid increased to 3.92 +/- 1.40 ng/ml and 13-cis-retinoic acid increased to 9.75 +/- 2.18 ng/ml. Maximal plasma 13-cis-4-oxoretinoic acid concentrations (average 7.60 +/- 1.45 ng/ml) were observed 6 h after dosing which was the last time point in this study. Concentrations of all-trans-4-oxoretinoic acid were low or not detectable. Our findings suggest that, in addition to all-trans-retinoic acid, 13-cis-retinoic acid and 13-cis-4-oxoretinoic acid are present in normal human plasma as metabolites of vitamin A.
C Eckhoff; H Nau
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  31     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1991-03-13     Completed Date:  1991-03-13     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1445-54     Citation Subset:  IM    
Institut für Toxikologie und Embryonalpharmakologie, Freie Universität Berlin, West Germany.
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MeSH Terms
Chromatography, Gas
Chromatography, High Pressure Liquid
Isotretinoin / blood*
Spectrophotometry, Ultraviolet
Tretinoin / analogs & derivatives*,  blood*
Vitamin A / administration & dosage,  pharmacology
Reg. No./Substance:
11103-57-4/Vitamin A; 302-79-4/Tretinoin; 334-88-3/Diazomethane; 38030-57-8/4-oxoretinoic acid; 4759-48-2/Isotretinoin

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