Document Detail


Identification of putative voltage-dependent Ca2+-permeable channels involved in cryptogein-induced Ca2+ transients and defense responses in tobacco BY-2 cells.
MedLine Citation:
PMID:  15081414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ca(2+) is the pivotal second messenger for induction of defense responses induced by treatment of pathogen-derived elicitor or microbial infection in plants. However, molecular bases for elicitor-induced generation of Ca(2+) signals (Ca(2+) transients) are largely unknown. We here identified cDNAs for putative voltage-dependent Ca(2+)-permeable channels, NtTPC1A and NtTPC1B, that are homologous to TPC1 (two pore channel) from suspension-cultured tobacco BY-2 cells. NtTPC1s complemented the growth of a Saccharomyces cerevisiae mutant defective in CCH1, a putative Ca(2+) channel, in a low Ca(2+) medium, suggesting that both products permeate Ca(2+) through the plasma membrane. Cosuppression of NtTPC1s in apoaequorin-expressing BY-2 cells resulted in inhibition of rise in cytosolic free Ca(2+) concentration ([Ca(2+)](cyt)) in response to sucrose and a fungal elicitor cryptogein, while it did not affect hypoosmotic shock-induced [Ca(2+)](cyt) increase. Cosuppression of NtTPC1s also caused suppression of cryptogein-induced programmed cell death and defense-related gene expression. These results suggest that NtTPC1s are involved in Ca(2+) mobilization induced by the cryptogein and sucrose, and have crucial roles in cryptogein-induced signal transduction pathway.
Authors:
Yasuhiro Kadota; Takuya Furuichi; Yoko Ogasawara; Tatsuaki Goh; Katsumi Higashi; Shoshi Muto; Kazuyuki Kuchitsu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  317     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-14     Completed Date:  2004-05-19     Revised Date:  2012-07-20    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  823-30     Citation Subset:  IM    
Affiliation:
Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
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MeSH Terms
Descriptor/Qualifier:
Algal Proteins / pharmacology*
Amino Acid Sequence
Base Sequence
Calcium / physiology*
Calcium Channels / chemistry,  drug effects,  genetics,  physiology*
Cell Line
Cloning, Molecular
DNA Primers
Molecular Sequence Data
Saccharomyces cerevisiae / growth & development
Sequence Homology, Amino Acid
Tobacco / cytology,  physiology*
Chemical
Reg. No./Substance:
0/Algal Proteins; 0/Calcium Channels; 0/DNA Primers; 0/cryptogein protein, Phytophthora cryptogea; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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