| Identification of a putative receptor for subgroup A feline leukemia virus on feline T cells. | |
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MedLine Citation:
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PMID: 1316477 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Retrovirus infection is initiated by the binding of virus envelope glycoprotein to a receptor molecule present on cell membranes. To characterize a receptor for feline leukemia virus (FeLV), we extensively purified the viral envelope glycoprotein, gp70, from culture supernatants of FeLV-61E (subgroup A)-infected cells by immunoaffinity chromatography. Binding of purified 125I-labeled gp70 to the feline T-cell line 3201 was specific and saturable, and Scatchard analysis revealed a single class of receptor binding sites with an average number of 1.6 x 10(5) receptors per cell and an apparent affinity constant (Ka) of 1.15 x 10(9) M-1. Cross-linking experiments identified a putative gp70-receptor complex of 135 to 140 kDa. Similarly, coprecipitation of 125I-labeled cell surface proteins with purified gp70 and a neutralizing but noninterfering anti-gp70 monoclonal antibody revealed a single cell surface protein of approximately 70 kDa. These results indicate that FeLV-A binds to feline T cells via a 70-kDa cell surface protein, its presumptive receptor. |
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Authors:
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A K Ghosh; M H Bachmann; E A Hoover; J I Mullins |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 66 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1992 Jun |
Date Detail:
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Created Date: 1992-06-16 Completed Date: 1992-06-16 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3707-14 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal Cats Cell Line Chromatography, Affinity Cross-Linking Reagents Kinetics Leukemia Virus, Feline / metabolism* Leukemia, Experimental / metabolism* Precipitin Tests Receptors, Virus / chemistry* Retroviridae Proteins, Oncogenic / immunology, isolation & purification, metabolism* T-Lymphocytes / cytology, metabolism* Viral Envelope Proteins / immunology, isolation & purification, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI25273/AI/NIAID NIH HHS; CA43216/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Cross-Linking Reagents; 0/Receptors, Virus; 0/Retroviridae Proteins, Oncogenic; 0/Viral Envelope Proteins; 0/feline leukemia virus receptor; 0/glycoprotein gp70, Feline leukemia virus |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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