Document Detail

Identification of a putative receptor for subgroup A feline leukemia virus on feline T cells.
MedLine Citation:
PMID:  1316477     Owner:  NLM     Status:  MEDLINE    
Retrovirus infection is initiated by the binding of virus envelope glycoprotein to a receptor molecule present on cell membranes. To characterize a receptor for feline leukemia virus (FeLV), we extensively purified the viral envelope glycoprotein, gp70, from culture supernatants of FeLV-61E (subgroup A)-infected cells by immunoaffinity chromatography. Binding of purified 125I-labeled gp70 to the feline T-cell line 3201 was specific and saturable, and Scatchard analysis revealed a single class of receptor binding sites with an average number of 1.6 x 10(5) receptors per cell and an apparent affinity constant (Ka) of 1.15 x 10(9) M-1. Cross-linking experiments identified a putative gp70-receptor complex of 135 to 140 kDa. Similarly, coprecipitation of 125I-labeled cell surface proteins with purified gp70 and a neutralizing but noninterfering anti-gp70 monoclonal antibody revealed a single cell surface protein of approximately 70 kDa. These results indicate that FeLV-A binds to feline T cells via a 70-kDa cell surface protein, its presumptive receptor.
A K Ghosh; M H Bachmann; E A Hoover; J I Mullins
Related Documents :
15896347 - Cell line dependent rna expression profiles of prion-infected mouse neuronal cells.
15242167 - Differential gene expression in individual papilla-resistant and powdery mildew-infecte...
10092677 - Selective perturbation of early endosome and/or trans-golgi network ph but not lysosome...
3881557 - Biochemical analysis of rabies virus proteins in three persistently infected bhk-21 cel...
21302257 - Isolation of teleost primordial germ cells using flow cytometry.
24346697 - The actin-binding protein profilin is required for germline stem cell maintenance and g...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  66     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-06-16     Completed Date:  1992-06-16     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3707-14     Citation Subset:  IM    
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibodies, Monoclonal
Cell Line
Chromatography, Affinity
Cross-Linking Reagents
Leukemia Virus, Feline / metabolism*
Leukemia, Experimental / metabolism*
Precipitin Tests
Receptors, Virus / chemistry*
Retroviridae Proteins, Oncogenic / immunology,  isolation & purification,  metabolism*
T-Lymphocytes / cytology,  metabolism*
Viral Envelope Proteins / immunology,  isolation & purification,  metabolism*
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Cross-Linking Reagents; 0/Receptors, Virus; 0/Retroviridae Proteins, Oncogenic; 0/Viral Envelope Proteins; 0/feline leukemia virus receptor; 0/glycoprotein gp70, Feline leukemia virus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Transcription from varicella-zoster virus gene 67 (glycoprotein IV).
Next Document:  Epstein-Barr virus latent gene expression in uncultured peripheral blood lymphocytes.