Document Detail


Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium.
MedLine Citation:
PMID:  19176476     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proteins that exhibit different expression levels in normal and malignant lung cells are good candidate biomarkers to improve early diagnosis and intervention. We used a quantitative approach and compared the proteome of microdissected cells from normal human bronchial epithelium and squamous cell carcinoma tumors of histopathological grades G2 and G3. DIGE analysis and subsequent MS-based protein identification revealed that 32 non-redundant proteins were differentially regulated between the respective tissue types. These proteins are mainly involved in energy pathways, cell growth or maintenance mechanisms, protein metabolism, and the regulation of DNA and RNA metabolism. The expression of some of these proteins was analyzed by immunohistochemistry using tissue microarrays containing tissue specimen of 55 patients, including normal bronchial epithelium, squamous cell carcinomas, adenocarcinomas, and large cell carcinomas. The results of the immunohistochemical studies correlated with the proteome study data and revealed that particularly HSP47 and a group of cytokeratins (i.e. cytokeratins 6a, 16, and 17) are significantly co-regulated in squamous cell carcinoma. Furthermore cytokeratin 17 showed significantly higher abundance in G2 grade compared with G3 grade squamous cell carcinomas in both the gel-based and the immunohistochemical analysis. Therefore this protein might be used as a marker for stratification between different tumor grades.
Authors:
Gereon Poschmann; Barbara Sitek; Bence Sipos; Anna Ulrich; Sebastian Wiese; Christian Stephan; Bettina Warscheid; Günter Klöppel; Ann Vander Borght; Frans C S Ramaekers; Helmut E Meyer; Kai Stühler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-27
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  8     ISSN:  1535-9484     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-04     Completed Date:  2009-06-24     Revised Date:  2010-09-22    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1105-16     Citation Subset:  IM    
Affiliation:
Medizinisches Proteom-Center, Ruhr-Universität Bochum, 44801 Bochum, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Bronchi / metabolism*
Carcinoma, Squamous Cell / metabolism*,  pathology
Cluster Analysis
Electrophoresis, Gel, Two-Dimensional
Epithelium / metabolism*
Female
Humans
Immunohistochemistry
Keratin-17 / metabolism
Lung Neoplasms / metabolism*,  pathology
Male
Microdissection
Middle Aged
Neoplasm Proteins / analysis
Proteome / analysis*
ROC Curve
Tissue Array Analysis
Chemical
Reg. No./Substance:
0/Keratin-17; 0/Neoplasm Proteins; 0/Proteome
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