| Identification of proteins responsible for the multiple drug resistance in 5-fluorouracil-induced breast cancer cell using proteomics analysis. | |
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MedLine Citation:
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PMID: 20700687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: This study aimed to explore the mechanism of multi-drug resistance (MDR) in 5-fluorouracil (5-FU)-induced breast cancer cell MCF-7. METHODS: MCF-7 cells were exposed in stepwise escalating concentration of 5-FU to develop the resistant cell line, MCF-7/5-FU. Biological and molecular characteristics of the cells were studied through MTT, flow cytometry, real-time PCR, western-blot, and the global protein profiles between MCF-7/5-FU and parental MCF-7 were compared using proteomic approach. Then some of the differentially expressed proteins were validated by western-blot. In addition, the role of 14-3-3sigma was validated using gene transfection. RESULTS: Drug resistance of MCF-7/5-FU cells to 5-FU, MX, cDDP, ADM, TAXOL all increased significantly compared with MCF-7 cells and that maybe related to BCRP, but not MDR1 and MRP1. Differentially expressed proteins between MCF-7/5-FU and MCF-7 cells were identified; 12 proteins were up-regulated and 18 proteins were down-regulated in MCF-7/5-FU cells. Expressive levels of some proteins in western-blot validation were consistent with the results in proteomic analysis. Enforced 14-3-3sigma expression can increase the sensitivity of MCF-7/5-FU cells to 5-FU and cDDP. CONCLUSION: MDR of MCF-7/5-FU likely associated with differentially expressed proteins and 14-3-3sigma may play a positive role in chemotherapy. These findings may provide theoretical support for the prediction of chemotherapeutic response and reverse of MDR. |
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Authors:
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Guopei Zheng; Fang Peng; Renkui Ding; Yanhui Yu; Yongmei Ouyang; Zhuchu Chen; Zhiqiang Xiao; Zhimin He |
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Publication Detail:
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Type: Journal Article Date: 2010-02-21 |
Journal Detail:
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Title: Journal of cancer research and clinical oncology Volume: 136 ISSN: 1432-1335 ISO Abbreviation: J. Cancer Res. Clin. Oncol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-11 Completed Date: 2010-09-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902060 Medline TA: J Cancer Res Clin Oncol Country: Germany |
Other Details:
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Languages: eng Pagination: 1477-88 Citation Subset: IM |
Affiliation:
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Cancer Research Institute, Xiangya School of Medicine, Central South University, Xiangya Road #110, Changsha 410078, Hunan, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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14-3-3 Proteins
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physiology Amino Acid Sequence Breast Neoplasms / chemistry, drug therapy*, pathology Cell Line, Tumor Drug Resistance, Multiple Drug Resistance, Neoplasm Exonucleases / physiology Female Fluorouracil / pharmacology* HSC70 Heat-Shock Proteins / physiology Humans Keratin-8 / physiology Molecular Sequence Data Neoplasm Proteins / analysis*, physiology Peptide Elongation Factor Tu / physiology Proteomics / methods* Superoxide Dismutase / physiology Tumor Markers, Biological / physiology |
| Chemical | |
Reg. No./Substance:
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0/14-3-3 Proteins; 0/HSC70 Heat-Shock Proteins; 0/HSPA8 protein, human; 0/Keratin-8; 0/Neoplasm Proteins; 0/Tumor Markers, Biological; 51-21-8/Fluorouracil; EC 1.15.1.1/Superoxide Dismutase; EC 1.15.1.1/superoxide dismutase 2; EC 3.1.-/Exonucleases; EC 3.1.-/SFN protein, human; EC 3.6.1.-/Peptide Elongation Factor Tu |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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