| Identification of proteins adsorbed from human plasma to glass bead columns: plasmin-induced degradation of adsorbed fibrinogen. | |
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MedLine Citation:
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PMID: 2946694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a bead column experiment, attempts have been made to identify the proteins adsorbed from plasma onto a glass surface. Proteins adsorbed after a 3-h contact time were eluted sequentially by 1 M tris buffer and SDS. Polyacrylamide gel electrophoresis of eluted proteins showed a multiplicity of components, and not all of these could be identified. Positive identifications were made by immunodiffusion against specific antibodies, band positions on electrophoresis gels, and location of radioactivity in gels when specific radiolabeled proteins were added to plasma. Proteins found were albumin, IgG, fibrinogen, plasminogen, and fibrinogen degradation products (FDP). A major component with an apparent molecular weight of 25,000 remains unidentified. It is unrelated to albumin, IgG, fibrinogen, factor XII, or plasminogen. Adsorbed fibrinogen was less degraded when experiments were performed with plasmas deficient in either plasminogen or factor XII. It is therefore concluded that FDP are formed by activation of adsorbed plasminogen, as was found previously for purified fibrinogen containing a trace of plasminogen. At least part of this activation is potentiated by the contact activation phase of plasma coagulation, in particular activated factor XII. |
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Authors:
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J L Brash; J A Thibodeau |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research Volume: 20 ISSN: 0021-9304 ISO Abbreviation: J. Biomed. Mater. Res. Publication Date: 1986 Nov-Dec |
Date Detail:
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Created Date: 1986-12-31 Completed Date: 1986-12-31 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0112726 Medline TA: J Biomed Mater Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1263-75 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Blood Proteins / isolation & purification, metabolism* Electrophoresis, Polyacrylamide Gel Enzymes, Immobilized / metabolism* Fibrinogen / metabolism* Fibrinolysin / metabolism* Glass Humans |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins; 0/Enzymes, Immobilized; 9001-32-5/Fibrinogen; EC 3.4.21.7/Fibrinolysin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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