Document Detail


Identification of a protein kinase gene associated with pistillody, homeotic transformation of stamens into pistil-like structures, in alloplasmic wheat.
MedLine Citation:
PMID:  17704940     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Homeotic transformation of stamens into pistil-like structures (called pistillody) has been reported in cytoplasmic substitution (alloplasmic) lines of bread wheat (Triticum aestivum) having the cytoplasm of a wild relative species, Aegilops crassa. Our previous studies indicated that pistillody is caused by alterations of the class B MADS-box gene expression pattern associated with mitochondrial gene(s) in the Ae. crassa cytoplasm. To elucidate the nuclear gene involved in the cross-talk between pistillody-related mitochondrial gene(s) and nuclear homeotic genes, we performed cDNA subtraction analysis using cDNAs derived from young spikes of a pistillody line and a normal line. As a result, we identified a protein kinase gene, WPPK1 (wheat pistillody-related protein kinase 1), which is upregulated in the young spikes of the pistillody line. RT-PCR analysis indicated that WPPK1 is strongly expressed in pistils and pistil-like stamens in the pistillody line, suggesting that it is involved in the formation of pistil-like stamens as well as pistils. The full-length cDNA sequence for WPPK1 showed high similarity with a flowering plant PVPK-1 protein kinase, and phylogenetic analysis indicated that it is a member of AGC group protein kinases. Furthermore, a phosphorylation assay indicated that it has protein kinase activity. In situ hybridization analysis revealed that WPPK1 is expressed in developing pistils and pistil-like stamens as well as in their primordia. These indicate that in the alloplasmic line, WPPK1 plays a role in formation and development of pistil-like stamens.
Authors:
Tatsunori Saraike; Naoki Shitsukawa; Yuko Yamamoto; Hiroko Hagita; Yukimoto Iwasaki; Shigeo Takumi; Koji Murai
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-18
Journal Detail:
Title:  Planta     Volume:  227     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-03-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  211-21     Citation Subset:  IM    
Affiliation:
Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuoka-Kenjojima, Yoshida-Gun, Fukui, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB298101
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Blotting, Western
Flowers / enzymology,  genetics*,  growth & development
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
In Situ Hybridization
Molecular Sequence Data
Phylogeny
Plant Proteins / genetics*,  metabolism
Protein Kinases / genetics*,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Triticum / enzymology,  genetics*,  growth & development
Chemical
Reg. No./Substance:
0/Plant Proteins; EC 2.7.-/Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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