| Identification of a protein kinase gene associated with pistillody, homeotic transformation of stamens into pistil-like structures, in alloplasmic wheat. | |
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MedLine Citation:
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PMID: 17704940 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Homeotic transformation of stamens into pistil-like structures (called pistillody) has been reported in cytoplasmic substitution (alloplasmic) lines of bread wheat (Triticum aestivum) having the cytoplasm of a wild relative species, Aegilops crassa. Our previous studies indicated that pistillody is caused by alterations of the class B MADS-box gene expression pattern associated with mitochondrial gene(s) in the Ae. crassa cytoplasm. To elucidate the nuclear gene involved in the cross-talk between pistillody-related mitochondrial gene(s) and nuclear homeotic genes, we performed cDNA subtraction analysis using cDNAs derived from young spikes of a pistillody line and a normal line. As a result, we identified a protein kinase gene, WPPK1 (wheat pistillody-related protein kinase 1), which is upregulated in the young spikes of the pistillody line. RT-PCR analysis indicated that WPPK1 is strongly expressed in pistils and pistil-like stamens in the pistillody line, suggesting that it is involved in the formation of pistil-like stamens as well as pistils. The full-length cDNA sequence for WPPK1 showed high similarity with a flowering plant PVPK-1 protein kinase, and phylogenetic analysis indicated that it is a member of AGC group protein kinases. Furthermore, a phosphorylation assay indicated that it has protein kinase activity. In situ hybridization analysis revealed that WPPK1 is expressed in developing pistils and pistil-like stamens as well as in their primordia. These indicate that in the alloplasmic line, WPPK1 plays a role in formation and development of pistil-like stamens. |
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Authors:
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Tatsunori Saraike; Naoki Shitsukawa; Yuko Yamamoto; Hiroko Hagita; Yukimoto Iwasaki; Shigeo Takumi; Koji Murai |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-08-18 |
Journal Detail:
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Title: Planta Volume: 227 ISSN: 0032-0935 ISO Abbreviation: Planta Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-06 Completed Date: 2008-03-13 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
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Languages: eng Pagination: 211-21 Citation Subset: IM |
Affiliation:
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Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuoka-Kenjojima, Yoshida-Gun, Fukui, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB298101 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Blotting, Western Flowers / enzymology, genetics*, growth & development Gene Expression Regulation, Developmental Gene Expression Regulation, Plant In Situ Hybridization Molecular Sequence Data Phylogeny Plant Proteins / genetics*, metabolism Protein Kinases / genetics*, metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Triticum / enzymology, genetics*, growth & development |
| Chemical | |
Reg. No./Substance:
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0/Plant Proteins; EC 2.7.-/Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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