Document Detail


Identification and prospective isolation of a mesothelial precursor lineage giving rise to smooth muscle cells and fibroblasts for mammalian internal organs, and their vasculature.
MedLine Citation:
PMID:  23143399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fibroblasts and smooth muscle cells (FSMCs) are principal cell types of connective and adventitial tissues that participate in the development, physiology and pathology of internal organs, with incompletely defined cellular origins. Here, we identify and prospectively isolate from the mesothelium a mouse cell lineage that is committed to FSMCs. The mesothelium is an epithelial monolayer covering the vertebrate thoracic and abdominal cavities and internal organs. Time-lapse imaging and transplantation experiments reveal robust generation of FSMCs from the mesothelium. By targeting mesothelin (MSLN), a surface marker expressed on mesothelial cells, we identify and isolate precursors capable of clonally generating FSMCs. Using a genetic lineage tracing approach, we show that embryonic and adult mesothelium represents a common lineage to trunk FSMCs, and trunk vasculature, with minimal contributions from neural crest, or circulating cells. The isolation of FSMC precursors enables the examination of multiple aspects of smooth muscle and fibroblast biology as well as the prospective isolation of these precursors for potential regenerative medicine purposes.
Authors:
Yuval Rinkevich; Taisuke Mori; Debashis Sahoo; Pin-Xian Xu; John R Bermingham; Irving L Weissman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-11
Journal Detail:
Title:  Nature cell biology     Volume:  14     ISSN:  1476-4679     ISO Abbreviation:  Nat. Cell Biol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  2013-01-28     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  100890575     Medline TA:  Nat Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1251-60     Citation Subset:  IM    
Affiliation:
Institute for Stem Cell Biology and Regenerative Medicine, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA. ryuval@stanford.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Epithelium / metabolism*
Fibroblasts / cytology*,  drug effects
Flow Cytometry
GPI-Linked Proteins / metabolism
Gastrointestinal Tract / cytology,  drug effects,  metabolism
Genotype
Immunohistochemistry
Mice
Mice, Transgenic
Myocytes, Smooth Muscle / cytology*,  drug effects
Tamoxifen / pharmacology
Grant Support
ID/Acronym/Agency:
R01 DK064640/DK/NIDDK NIH HHS; R01 DK064640/DK/NIDDK NIH HHS; R01 NS040751/NS/NINDS NIH HHS; U01 HL099999/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/GPI-Linked Proteins; 0/mesothelin; 10540-29-1/Tamoxifen
Comments/Corrections

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