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Identification of promiscuous HLA-DR-restricted CD4(+) T-cell epitopes on the cancer-testis antigen HCA587.
MedLine Citation:
PMID:  21595801     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The cancer testis antigen HCA587 is an attractive candidate for T cell-based immunotherapy because it is overexpressed in a wide spectrum of malignant tumors, but not normal tissues except testis. Several CTL epitopes derived from HCA587 have been described. Our goal was to identify helper T lymphocyte epitopes of HCA587 for the optimization of T cell-based immunotherapies against HCA587-expressing tumors. Candidate helper T lymphocyte epitopes for HCA587 were predicted using SYFPEITHI algorithm and were tested for their ability to induce helper T lymphocyte responses by in vitro peptide vaccination of CD4(+) T lymphocytes from healthy individuals and hepatocellular carcinoma patients. Four CD4(+) T-cell epitopes for HCA587 (p43-57, p145-159, p186-200 and p249-263) were identified. Among them, the p43-57 epitope was shown to be naturally processed and presented by HCA587-expressing tumor cells as well as autologous dendritic cells pulsed with whole-protein HCA587. Notably, this epitope behaved as a promiscuous T-cell epitope as it stimulated T cells in the context of more than one HLA class II allele. Thus, p43-57 is the first HCA587-derived MHC class II-restricted epitope to fulfill all prerequisites for use as a peptide vaccine in patients with HCA587-expressing tumors.
Authors:
Weigang Wen; Lijie Zhang; Jirun Peng; Juanjuan Chen; Jiaqing Hao; Xiaofeng Li; Xiaoping Qian; Pumei Zeng; Yu Zhang; Yanhui Yin
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-19
Journal Detail:
Title:  Cancer science     Volume:  -     ISSN:  1349-7006     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101168776     Medline TA:  Cancer Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Japanese Cancer Association.
Affiliation:
Department of Immunology, Peking University Health Science Center, 38 Xue Yuan Road, Haidian District, Beijing 100191, China Center of Hepatobiliary Surgery, People's Hospital, Peking University Health Science Center, No. 11 Xizhimen Street, Beijing 100044, China.
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