Document Detail


Identification and profiling of targeted oxidized linoleic acid metabolites in rat plasma by quadrupole time-of-flight mass spectrometry.
MedLine Citation:
PMID:  23037960     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Linoleic acid (LA) and LA-esters are the precursors of LA hydroperoxides, which are readily converted to 9- and 13-hydroxy-​octadecadienoic acid (HODE) and 9- and 13-oxo-​octadecadienoic acid (oxo ODE) metabolites in vivo. These four oxidized LA metabolites (OXLAMs) have been implicated in a variety of pathological conditions. Therefore, their accurate measurement may provide mechanistic insights into disease pathogenesis. Here we present a novel quadrupole time-of-flight mass spectrometry (Q-TOFMS) method for quantitation and identification of target OXLAMs in rat plasma. In this method, the esterified OXLAMs were base-hydrolyzed and followed by liquid-liquid extraction. Quantitative analyses were based on one-point standard addition with isotope dilution. The Q-TOFMS data of target metabolites were acquired and multiple reaction monitoring extracted-ion chromatograms were generated post-acquisition with a 10 ppm extraction window. The limit of quantitation was 9.7-35.9 nmol/L depending on the metabolite. The method was reproducible with a coefficient of variation of <18.5%. Mean concentrations of target metabolites in rat plasma were 57.8, 123.2, 218.1 and 57.8 nmol/L for 9-HODE, 13-HODE, 9-oxoODE and 13-oxoODE, respectively. Plasma levels of total OXLAMs were 456.9 nmol/L, which correlated well with published concentrations obtained by gas chromatography/mass spectrometry (GC/MS). The concentrations were also obtained utilizing a standard addition curve approach. The calibration curves were linear with correlation coefficients of >0.991. Concentrations of 9-HODE, 13-HODE, 9-oxoODE and 13-oxoODE were 84.0, 138.6, 263.0 and 69.5 nmol/L, respectively, which were consistent with the results obtained from one-point standard addition. Target metabolites were simultaneously characterized based on the accurate Q-TOFMS data. This is the first study of secondary LA metabolites using Q-TOFMS. Published 2012. This article is a U.S. Government work and is in the public domain in the USA.
Authors:
Zhi-Xin Yuan; Stanley I Rapoport; Steven J Soldin; Alan T Remaley; Ameer Y Taha; Matthew Kellom; Jianghong Gu; Maureen Sampson; Christopher E Ramsden
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Intramural     Date:  2012-10-05
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  27     ISSN:  1099-0801     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-06     Completed Date:  2013-08-21     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  England    
Other Details:
Languages:  eng     Pagination:  422-32     Citation Subset:  IM    
Copyright Information:
Published 2012. This article is a U.S. Government work and is in the public domain in the USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Liquid / methods
Limit of Detection
Linoleic Acids / blood*
Linoleic Acids, Conjugated / blood*
Linolenic Acids / blood*
Rats
Reproducibility of Results
Tandem Mass Spectrometry / methods*
Grant Support
ID/Acronym/Agency:
Z01 AG000399-04/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Linoleic Acids; 0/Linoleic Acids, Conjugated; 0/Linolenic Acids; 15514-85-9/9-hydroxy-10,12-octadecadienoic acid; 31385-09-8/13-oxo-9,11-octadecadienoic acid; 5204-88-6/13-hydroxy-9,11-octadecadienoic acid; 54665-32-6/9-oxo-10,12-octadecadienoic acid
Comments/Corrections

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