| Identification of problems developing an ultrasensitive immunoassay for the ante mortem detection of the infectious isoform of the CWD-associated prion protein. | |
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MedLine Citation:
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PMID: 19330640 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ante-mortem assays exist for some Transmission Spongiform Encephalopathies (TSE). These assays facilitate our understanding of disease pathology and epidemiology; however, the limitations of these ante-mortem assays include the inability to quantify protein amount, poor sensitivity, and/or limited robustness. Here, we utilize a bioinformatics approach to report on problems associated with developing a more sensitive immunoassay for TSEs including: 1) the lack of specific and sufficiently sensitive antibodies for the infectious isoform(s) of PrP(res), 2) problems associated with serial titration of PrP(res), and 3) the distribution of PrP(res) particle sizes. Overcoming these problems require more sophisticated antibody design and a creative engineering of an ultrasensitive protein assay systems for PrP(res). |
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Authors:
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Benjamin Brooks; Amanda Brooks; Shaun S Wulff; Randolph V Lewis |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of immunoassay & immunochemistry Volume: 30 ISSN: 1532-4230 ISO Abbreviation: J Immunoassay Immunochem Publication Date: 2009 |
Date Detail:
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Created Date: 2009-03-30 Completed Date: 2009-05-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100963688 Medline TA: J Immunoassay Immunochem Country: United States |
Other Details:
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Languages: eng Pagination: 135-49 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA. bbrooks@uwyo.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Computational Biology / methods* Computer Simulation Immunoassay / standards* Prions / analysis* Protein Isoforms / analysis Sensitivity and Specificity Wasting Disease, Chronic / diagnosis*, immunology |
| Chemical | |
Reg. No./Substance:
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0/Prions; 0/Protein Isoforms |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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