| Identification and preliminary characterization of ubiquitin C terminal hydrolase 1 (UCHL1) as a biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage. | |
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MedLine Citation:
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PMID: 20077430 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By using two different approaches, ubiquitin C-terminal hydrolase 1 (UCHL1) was identified as a potential cerebrospinal fluid (CSF) biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage (ASAH) and presumably other CNS damage and disease states. Appropriate antibodies and a sensitive ELISA were generated, and the release of UCHL1 into CSF was compared with that of pNF-H and S100beta in a cohort of 30 ASAH patients. Both UCHL1 and pNF-H showed persistent release into CSF in almost all patients in the second week postaneurysmal rupture (AR), and S100beta levels rapidly declined to baseline levels in 23 of 30 patients. Seven of thirty patients showed persistently elevated S100beta levels over the first 5 days post-AR and also had relatively higher levels of pNF-H and UCHL1 higher compared with the rest. These patients proved to have very poor outcomes, with 6 of 7 expiring. Patients who did reduce S100beta levels tended to have a better outcome if pNF-H and UCHL1 levels were also lower, and elevated UCHL1 levels in the second week post-AR were particularly predictive of poor outcome. Acute coordinated releases of large amounts of UCHL1, pNF-H, and S100beta in 16 of 30 patients were observed, suggesting sudden loss of brain tissues associated with secondary events. We conclude that measurement of the CSF levels of these proteins reveals details of ASAH progression and recovery and predicts patient outcome. |
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Authors:
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Stephen B Lewis; Regina Wolper; Yueh-Yun Chi; Lynn Miralia; Yong Wang; Cui Yang; Gerry Shaw |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neuroscience research Volume: 88 ISSN: 1097-4547 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-08 Completed Date: 2010-06-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: United States |
Other Details:
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Languages: eng Pagination: 1475-84 Citation Subset: IM |
Copyright Information:
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(c) 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Neurological Surgery, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, FL 32610, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Biological Markers / analysis, cerebrospinal fluid Brain Infarction / cerebrospinal fluid, diagnosis, enzymology Cohort Studies Enzyme-Linked Immunosorbent Assay Female Humans Male Middle Aged Nerve Degeneration / cerebrospinal fluid*, diagnosis, enzymology* Nerve Growth Factors / analysis, cerebrospinal fluid Neurofilament Proteins / analysis, cerebrospinal fluid Neurons / enzymology*, pathology Predictive Value of Tests Prognosis S100 Proteins / analysis, cerebrospinal fluid Subarachnoid Hemorrhage / complications* Ubiquitin Thiolesterase / analysis, cerebrospinal fluid* Up-Regulation / physiology |
| Grant Support | |
ID/Acronym/Agency:
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M01-RR00082/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Nerve Growth Factors; 0/Neurofilament Proteins; 0/S-100 calcium-binding protein beta subunit; 0/S100 Proteins; 108688-71-7/neurofilament protein H; EC 3.1.2.15/UCHL1 protein, human; EC 3.1.2.15/Ubiquitin Thiolesterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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