| Identification of potential vaccine target antigens by immunoproteomic analysis of a virulent and a non-virulent strain of the fish pathogen Flavobacterium psychrophilum. | |
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MedLine Citation:
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PMID: 17425262 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Flavobacterium psychrophilum is the etiological agent of bacterial coldwater disease (CWD) and rainbow trout fry syndrome (RTFS). To identify antigens associated with virulence or host immunity, we compared total and immunogenic proteins of cellular and extracellular products (ECP) between a virulent (CSF-259-93) and non-virulent (ATCC 49418) strain of F. psychrophilum. One-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis of total cellular proteins revealed only minor differences between the strains; however, separation of ECP showed that proteins were differentially expressed. Western blot analysis using rainbow trout (Oncorhynchus mykiss) anti-CSF-259-93 sera showed greater reactivity to proteins of the virulent strain, including many > 50 kDa. Further analysis by 2-dimensional electrophoresis (2DE) identified numerous differences between the strains. Western blot analysis combined with 2DE identified several immunogenic proteins that reacted with the antisera and were shared between the 2 strains. However, at least 15 immunogenic proteins appeared to be unique to the virulent strain, while 4 such proteins were identified in the non-virulent strain; 8 proteins unique to the virulent strain and 6 shared proteins were further analyzed for identification by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis. Of these, 3 immunogenic proteins (heat shock proteins HSP 60 and HSP 70) and 2 other proteins (ATP synthase and thermolysin) were conclusively identified. The 2 highly immunogenic heat shock proteins were shown to share extensive homology with heat shock proteins of related bacteria. This approach for antigen identification may provide a basis for targeted vaccine development against CWD and RTFS. |
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Authors:
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Ponnerassery S Sudheesh; Benjamin R LaFrentz; Douglas R Call; William F Siems; Scott E LaPatra; Gregory D Wiens; Kenneth D Cain |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Diseases of aquatic organisms Volume: 74 ISSN: 0177-5103 ISO Abbreviation: Dis. Aquat. Org. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-04-11 Completed Date: 2007-06-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8807037 Medline TA: Dis Aquat Organ Country: Germany |
Other Details:
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Languages: eng Pagination: 37-47 Citation Subset: IM |
Affiliation:
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Department of Fish and Wildlife Resources,e Aquaculture Research Institute, University of Idaho, Moscow, Idaho 83844-1136, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY955285; AY955286 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Bacterial / analysis* Bacterial Proteins / genetics Blotting, Western Chaperonin 60 / genetics Chromatography, Liquid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fish Diseases / microbiology* Flavobacteriaceae Infections / microbiology, veterinary* Flavobacterium / genetics, immunology, pathogenicity* Gene Expression / physiology Immune Sera / metabolism Immunoproteins / analysis*, isolation & purification Molecular Sequence Data Proteomics / methods Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Tandem Mass Spectrometry Virulence |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Chaperonin 60; 0/Immune Sera; 0/Immunoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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