| Identification of a polar targeting determinant for Bacillus subtilis DivIVA. | |
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MedLine Citation:
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PMID: 15554965 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Bacillus subtilis protein DivIVA controls both the positioning of the vegetative cell division site and the polar attachment of the chromosome during sporulation. In vegetative growth DivIVA attracts the bipartite cell division inhibitor MinCD away from the cell centre and towards the cell pole. This process ensures the inactivation of old polar division sites and leaves the cell centre free for the assembly of a new cell division complex. During sporulation MinCD and DivIVA levels fall, but DivIVA remains at the cell poles and becomes involved in the migration of the chromosomes to the pole. In order to investigate polar targeting of DivIVA, we undertook a mutational analysis of the 164-amino-acid protein. These studies identified one mutant (divIVA(R18C)) that could not localize to the cell pole but which retained the ability to support both vegetative growth and 50% sporulation efficiency. Further analysis revealed that, in the absence of polar targeting, DivIVA(R18C) localized to the nucleoid during vegetative growth in a Spo0J/Soj-dependent manner and required Spo0J/Soj and MinD to orientate the chromosomes correctly during sporulation. We demonstrate that polar targeting of DivIVA(R18C) is not essential during vegetative growth because the mutant can recognize the cell division site and influences the localization of MinD. Similarly we show that DivIVA(R18C) can function during sporulation because it can support the Spo0J/Soj orientation of the chromosome. In addition, we establish that both residues 18 and 19 constitute a DivIVA polar targeting determinant. |
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Authors:
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S E Perry; D H Edwards |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular microbiology Volume: 54 ISSN: 0950-382X ISO Abbreviation: Mol. Microbiol. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-11-23 Completed Date: 2005-04-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1237-49 Citation Subset: IM |
Affiliation:
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Division of Pathology and Neuroscience, University of Dundee, Ninewells Medical School, Dundee DD1 9SY, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Substitution Bacillus subtilis / chemistry, genetics*, metabolism Bacterial Proteins / genetics*, metabolism, physiology Cell Cycle Proteins / genetics*, metabolism Chromosomes, Bacterial / metabolism DNA Mutational Analysis Genes, Bacterial Mutagenesis Mutation Mutation, Missense Protein Sorting Signals / genetics*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Cell Cycle Proteins; 0/DivIVA protein, bacteria; 0/Protein Sorting Signals; 0/spore-specific proteins, Bacillus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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