Document Detail


Identification and physiological characterization of the nitrogen fixing bacterium Corynebacterium autotrophicum GZ 29.
MedLine Citation:
PMID:  1275646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The coryneform hydrogen bacterium strain GZ 29, assigned to Corynebacterium autotrophicum fixed molecular nitrogen under autotrophic (H2, CO2) as well as under heterotrophic (sucrose) conditions. Physiological parameters of nitrogen fixation were measured under heterotrophic conditions. The optimal dissolved oxygen concentration for cells grown in a fermenter with N2 was rather low (0.14 mg O2/l) compared with cells grown in the presence of NH+4 (4.45 mg O2/l). C. autotrophicum GZ 29 had a doubling time of 3.7 h at 30 degrees C with N2 as N-source and sucrose as carbon source and at optimal pO2. Acetylene reduction reached values of 12 nmoles of ethylene produced/min X mg protein. Although the oxygen concentration in the growing culture was kept constant, the optimal dissolved oxygen tension for the acetylene reduction assay shifted to higher pO2-values. The overall efficiency of nitrogen fixation amounted to 22 mg N fixed/g sucrose consumed; it reached a maximal value of 65 mg N fixed/g sucrose consumed at the beginning of the exponential growth phase. Intact cells reduced acetylene even under anaerobic test conditions; further anaerobic metabolic activity could not be ascertained so far.
Authors:
H Berndt; K P Ostwal; J Lalucat; C Schumann; F Mayer; H G Schlegel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of microbiology     Volume:  108     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  1976 May 
Date Detail:
Created Date:  1976-08-02     Completed Date:  1976-08-02     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  17-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylene / metabolism
Aerobiosis
Corynebacterium / classification,  growth & development,  isolation & purification*,  metabolism
Nitrogen / metabolism
Nitrogen Fixation*
Nitrogenase / analysis
Oxygen Consumption
Partial Pressure
Quaternary Ammonium Compounds / metabolism
Sucrose / metabolism
Chemical
Reg. No./Substance:
0/Quaternary Ammonium Compounds; 57-50-1/Sucrose; 74-86-2/Acetylene; 7727-37-9/Nitrogen; EC 1.18.6.1/Nitrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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