Document Detail


Identification of phospholipase C gamma1 as a protein tyrosine phosphatase mu substrate that regulates cell migration.
MedLine Citation:
PMID:  20506511     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The receptor protein tyrosine phosphatase PTPµ has a cell-adhesion molecule-like extracellular segment and a catalytically active intracellular segment. This structure gives PTPµ the ability to transduce signals in response to cell-cell adhesion. Full-length PTPµ is down-regulated in glioma cells by proteolysis which is linked to increased migration of these cells in the brain. To gain insight into the substrates PTPµ may be dephosphorylating to suppress glioma cell migration, we used a substrate trapping method to identify PTPµ substrates in tumor cell lines. We identified both PKCδ and PLCγ1 as PTPµ substrates. As PLCγ1 activation is linked to increased invasion of cancer cells, we set out to determine whether PTPµ may be upstream of PLCγ1 in regulating glioma cell migration. We conducted brain slice assays using U87-MG human glioma cells in which PTPµ expression was reduced by shRNA to induce migration. Treatment of the same cells with PTPµ shRNA and a PLCγ1 inhibitor prevented migration of the cells within the brain slice. These data suggest that PLCγ1 is downstream of PTPµ and that dephosphorylation of PLCγ1 is likely to be a major pathway through which PTPµ suppresses glioma cell migration.
Authors:
Polly J Phillips-Mason; Harpreet Kaur; Susan M Burden-Gulley; Sonya E L Craig; Susann M Brady-Kalnay
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  112     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-27     Completed Date:  2011-07-19     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  39-48     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, OH 44106-4960, USA.
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MeSH Terms
Descriptor/Qualifier:
Brain Neoplasms / metabolism
Cell Adhesion
Cell Line, Tumor
Cell Movement*
Glioma / metabolism
Humans
Phospholipase C gamma / genetics,  metabolism*
Phosphorylation
RNA, Messenger / metabolism
Receptor-Like Protein Tyrosine Phosphatases, Class 2 / genetics,  metabolism*
Signal Transduction
Grant Support
ID/Acronym/Agency:
P30 EY011373/EY/NEI NIH HHS; P30 EY011373-119002/EY/NEI NIH HHS; P30 EY011373-129002/EY/NEI NIH HHS; P30 EY011373-139002/EY/NEI NIH HHS; P30 EY011373-149002/EY/NEI NIH HHS; P30-EY11373/EY/NEI NIH HHS; R01 NS051520/NS/NINDS NIH HHS; R01 NS051520-01A1/NS/NINDS NIH HHS; R01 NS051520-02/NS/NINDS NIH HHS; R01 NS051520-03/NS/NINDS NIH HHS; R01 NS051520-04/NS/NINDS NIH HHS; R01 NS051520-05/NS/NINDS NIH HHS; R01-NS051520/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 3.1.3.48/Receptor-Like Protein Tyrosine Phosphatases, Class 2; EC 3.1.4.3/Phospholipase C gamma
Comments/Corrections

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