Document Detail


Identification of a peptide mimicking the binding pattern of an antiphospholipid antibody.
MedLine Citation:
PMID:  17015144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our objective was to characterize monoclonal antiphospholipid antibodies (APL) and identify disease-associated antigens in patients with the antiphospholipid syndrome (APS). We used the monoclonal antibody HL-5B, derived from a patient with APS suffering from multiple ischemic events, to screen a 12-mer peptide phage display library (New England Biolabs, London, England). The identified phage clones were sequenced and the derived consensus peptide was synthesized. The peptide was used to perform competitive inhibition experiments for their ability to inhibit the binding of the monoclonal antibody and of serum antibodies to cardiolipin and phosphatidylserine. Additionally patients and control sera were screened for their binding reactivities to this peptide. Using this 12-mer phage display library the peptide APHKHKASLSIY as consensus peptide for the monoclonal antiphospholipid antibody HL-5B could be identified. In competitive inhibition studies we showed that this peptide is able to inhibit the binding of HL-5B to cardiolipin and phosphatidylserine and furthermore another antiphospholipid antibody used as control was also inhibited in its binding to phospholipids. Using 21 sera from APS patients 67% showed a binding to the peptide in a specific ELISA above the cutoff level, generated with sera from 20 healthy controls. Out of the reactive patients' sera we used two exemplarily to perform inhibition studies. Both sera could be inhibited more than 40% in their binding to cardiolipin in a commercially available antiphospholipid antibody assay (Aescu.diagnostics, Wendelsheim, Germany). The identified peptide APHKHKASLSIY simulates the antigenic structure recognized from a subpopulation of serum antiphospholipid antibodies. This might indicate that the diversity of the antiphospholipid antibodies is limited and only few epitopes or few common structures are responsible for the development of those antibodies. Tests using these epitopes will strongly improve laboratory diagnosis of the APS.
Authors:
Christian Fischer; Katharina Buschmann; Miri Blank; Angelika Stachl; Wolfgang Miesbach; Yehuda Shoenfeld; Karl J Lackner; Philipp von Landenberg
Related Documents :
24339924 - Evaluation of anti-a/udorn/307/1972 antibody specificity to influenza a/h3n2 viruses us...
1795324 - Clinical utility and specificity of anticardiolipin antibodies.
1851504 - Antiphospholipid, antinuclear, epstein-barr and cytomegalovirus antibodies, and soluble...
20701624 - Evaluation of pg-m3 antibody in the diagnosis of acute promyelocytic leukaemia.
7014714 - Use of a specific antiserum for renin detection in human kidney.
9839554 - Antigen mimicry by an anti-idiotypic antibody single chain variable fragment.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-07
Journal Detail:
Title:  Immunobiology     Volume:  211     ISSN:  0171-2985     ISO Abbreviation:  Immunobiology     Publication Date:  2006  
Date Detail:
Created Date:  2006-10-03     Completed Date:  2006-12-01     Revised Date:  2007-01-11    
Medline Journal Info:
Nlm Unique ID:  8002742     Medline TA:  Immunobiology     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  695-9     Citation Subset:  IM    
Affiliation:
Institute of Clinical Chemistry and Laboratory Medicine, Johannes Gutenberg University, Mainz, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Amino Acid Sequence
Antibodies, Antiphospholipid / blood,  immunology*
Antibodies, Monoclonal / immunology*
Antibody Specificity*
Antiphospholipid Syndrome / blood,  immunology*
Enzyme-Linked Immunosorbent Assay
Female
Humans
Male
Middle Aged
Molecular Mimicry
Molecular Sequence Data
Peptide Library
Peptides / immunology*
Protein Binding
Chemical
Reg. No./Substance:
0/Antibodies, Antiphospholipid; 0/Antibodies, Monoclonal; 0/Peptide Library; 0/Peptides
Comments/Corrections
Erratum In:
Immunobiology. 2006;211(10):821

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Antiphospholipid antibodies as a possible risk factor for atherosclerosis in patients with rheumatoi...
Next Document:  Age-dependent loss of naïve T cells in TCR transgenic bone marrow chimeras.