| Identification and partial purification of calmodulin-binding microtubule-associated proteins from Neurospora crassa. | |
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MedLine Citation:
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PMID: 8001548 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have purified microtubule-associated proteins from Neurospora crassa on the basis of heat stability and affinity to calmodulin. Two proteins of molecular masses 170 kDa and 190 kDa have been partially purified. A third protein of 145 kDa was purified almost to homogeneity, and we present evidence that this protein is a specific substrate for a Ca2+/calmodulin-dependent protein kinase. The purified 170-, 190-, and 145-kDa proteins induce the assembly of microtubules from purified porcine brain tubulin. We demonstrate that all three proteins are microtubule-associated proteins on the basis of an in vitro microtubule-binding assay. |
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Authors:
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R Ortega Perez; I Irminger-Finger; J F Arrighi; N Capelli; D van Tuinen; G Turian |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 226 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1994 Dec |
Date Detail:
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Created Date: 1995-01-26 Completed Date: 1995-01-26 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY |
Other Details:
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Languages: eng Pagination: 303-10 Citation Subset: IM |
Affiliation:
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Laboratory of General Microbiology, University of Geneva, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies
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immunology Antibody Specificity Blotting, Western Calcium-Calmodulin-Dependent Protein Kinases / metabolism Calmodulin / metabolism* Chromatography, Affinity Drug Stability Hot Temperature Microscopy, Electron Microtubule-Associated Proteins / chemistry, isolation & purification*, metabolism Microtubules / metabolism, ultrastructure Neurospora crassa / chemistry* Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Calmodulin; 0/Microtubule-Associated Proteins; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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