Document Detail


Identification and partial characterization of a prolactin-like hormone produced by rat decidual tissue.
MedLine Citation:
PMID:  2982145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies have strongly, but indirectly, suggested that rat decidual tissue produces a prolactin-like hormone, decidual luteotropin, which markedly affects luteal cell function. However, it was also found that extracts of decidual tissue do not cross-react with antisera to either rat or ovine prolactin (PRL). The purpose of this study was to determine whether the decidual tissue contains a substance that binds to PRL receptors in rat luteal membranes and, if so, to identify, quantitate, and characterize this molecule with the use of an ovarian radioreceptor assay. Decidual tissue was induced in day 5 pseudopregnant rats by scratching the antimesometrial wall of the uterus; it was collected on day 9 and homogenized and extracted. Decidual tissue extracts bound specifically to ovarian PRL receptors. Graded dilutions of the extracts yielded curves that were parallel to the ovine PRL standard, indicating that decidual luteotropin competes for the same receptor sites on rat luteal membranes. To determine the levels of decidual luteotropin throughout pseudopregnancy, decidual tissue was obtained on each day between days 6-12. The PRL-like activity was detectable in decidual tissue as early as day 6, reached a maximum on day 9, and declined thereafter. The elution profile obtained from gel filtration of a day 9 decidual tissue extract displayed a major component of decidual luteotropin eluting at a Ve/Vo ratio of approximately equal to 2.0. Column chromatography indicated that decidual luteotropin corresponds to a protein with a molecular weight of 23,500. The hormone was heat labile, digestible by trypsin, and appears to contain disulfide linkages. In summary, this study reports the identification, quantitation, and partial characterization of a PRL-like hormone produced by the decidual tissue of the rat.
Authors:
P G Jayatilak; L A Glaser; R Basuray; P A Kelly; G Gibori
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  82     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1985 Jan 
Date Detail:
Created Date:  1985-02-27     Completed Date:  1985-02-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  217-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Decidua / physiology*
Dithiothreitol / pharmacology
Female
Molecular Weight
Ovary / metabolism
Pregnancy
Pregnancy, Animal*
Prolactin / analysis,  biosynthesis*
Rats
Receptors, Cell Surface / metabolism
Receptors, Prolactin
Temperature
Grant Support
ID/Acronym/Agency:
HD12356/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Cell Surface; 0/Receptors, Prolactin; 3483-12-3/Dithiothreitol; 9002-62-4/Prolactin
Comments/Corrections

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