| Identification and partial characterization of a lactogen from the midpregnant mouse conceptus. | |
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MedLine Citation:
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PMID: 6832047 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previously, we demonstrated that measurement of serum lactogenic hormones during pregnancy in the mouse by the rabbit mammary gland radioreceptor assay (RRA) and specific RIAs for mouse PRL (mPRL) and mouse placental lactogen (mPL) showed a large RRA to RIA activity ratio during midpregnancy. The purpose of this study was to begin to characterize this discrepancy in midpregnancy PRL-like activity. The elution profile of serum from midpregnant mice on Sephadex G-100 showed a characteristic peak in RRA activity (elution volume/void volume = 1.5), distinct from either mPRL or mPL (both approximately elution volume/void volume = 2.0). This PRL-like activity was not recognized by antisera to either mPRL or mPL. The source of the midpregnancy lactogen was localized to the conceptus. The hormone was found to have an apparent molecular weight of 50,000 (based on Sephadex G-100 chromatography) and an electrophoretic mobility similar to mPRL (Rf = 0.5-0.6) and to be lactogenic in the in vitro mouse mammary gland explant bioassay. We have demonstrated the existence of a midpregnancy lactogen that is different from mPL and mPRL and is of conceptus origin. |
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Authors:
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M J Soares; P Colosi; L Ogren; F Talamantes |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 112 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1983 Apr |
Date Detail:
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Created Date: 1983-05-05 Completed Date: 1983-05-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1313-7 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Gel Cross Reactions Embryo, Mammalian / analysis* Female Mice Mice, Inbred C3H Molecular Weight Pregnancy Prolactin / analysis* Radioimmunoassay Radioligand Assay |
| Grant Support | |
ID/Acronym/Agency:
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HD-14966/HD/NICHD NIH HHS; RR-08132/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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9002-62-4/Prolactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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