Document Detail


Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.
MedLine Citation:
PMID:  8022818     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Myosin diversity in the human epithelial cell line Caco-2BBe, the porcine epithelial cell line LLC-PK1 (CL-4), human peripheral blood leukocytes, and human liver was analyzed. PCR amplification yielded 8-11 putative myosins (depending on the cDNA source) representing six distinct myosin classes. Analysis of clones obtained by hybridization screening demonstrated that the original PCR products correspond to bona fide myosins, based on the presence of sequences highly conserved in other myosins. RNase protection analysis confirmed mRNA expression of 11 myosins in Caco-2BBe cells. Immunoblot analysis showed that at least 6 myosin immunogens are expressed in Caco-2BBe cells. The results reveal the existence of at least 11 unconventional human myosin genes, most of which are expressed in an overlapping fashion in different cell types. The abundance of myosins suggests that the myosin I vs. myosin II paradigm is inadequate to explain actin-based cellular motility.
Authors:
W M Bement; T Hasson; J A Wirth; R E Cheney; M S Mooseker
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  91     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-08-01     Completed Date:  1994-08-01     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6549-53     Citation Subset:  IM    
Affiliation:
Department of Biology, Yale University, New Haven, CT 06520-8103.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/L29128;  L29129;  L29130;  L29131;  L29132;  L29133;  L29134;  L29135;  L29136;  L29137;  L29138;  L29139;  L29140;  L29141;  L29142;  L29143;  L29144;  L29145;  L29146;  L29147;  L29148;  L29149
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Consensus Sequence*
DNA Primers
Epithelium / metabolism
Gene Expression*
Gene Library
Humans
Leukocytes / metabolism
Liver / metabolism
Molecular Sequence Data
Myosins / biosynthesis*,  genetics*
Polymerase Chain Reaction
Sequence Homology, Amino Acid
Swine
Vertebrates / metabolism*
Grant Support
ID/Acronym/Agency:
DK 25387/DK/NIDDK NIH HHS; DK 34989/DK/NIDDK NIH HHS; DK 38979/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; EC 3.6.4.1/Myosins
Comments/Corrections
Erratum In:
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11767   [PMID:  7972138 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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