| Identification of osteoclasts in culture. | |
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MedLine Citation:
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PMID: 21042999 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte-macrophage lineage. Bone-forming osteoblasts play a role in the formation of osteoclasts. Osteoblasts/stromal cells express two cytokines essential for osteoclastogenesis: receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Using RANKL and M-CSF, osteoclasts can be induced from monocyte-macrophage lineage cells even in the absence of osteoblasts. We describe here methods for the identification of osteoclasts formed in vitro. |
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Authors:
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Nobuyuki Udagawa; Teruhito Yamashita; Yasuhiro Kobayashi; Naoyuki Takahashi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 690 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2011 |
Date Detail:
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Created Date: 2010-11-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 273-84 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Matsumoto Dental University, Nagano, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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