Document Detail

Identification of an optimal Ncx binding sequence required for transcriptional activation.
MedLine Citation:
PMID:  10869550     Owner:  NLM     Status:  MEDLINE    
The Ncx gene encodes a homeobox containing transcription factor that belongs to the Hox11 gene family. We determined specific Ncx protein binding consensus DNA sequences. Optimal Ncx binding sequences were 5'-CGGTAATTGG-3' (TAAT core) and 5'-CGGTAAGTGG-3' (TAAG core), which coincided with the Hox11 binding sequence. Both Ncx and Hox11 could bind to the TAAT and the TAAG core oligonucleotide in vitro. However, they could efficiently transactivate the reporter plasmid linked to the TAAT core sequence but not to the TAAG core sequence. Thus, Ncx and Hox11 act as transcriptional activators via their target sequence, 5'-CGGTAATTGG-3'.
H Shimizu; M Kang; Y Iitsuka; M Ichinose; T Tokuhisa; M Hatano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  475     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-24     Completed Date:  2000-07-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  170-4     Citation Subset:  IM    
Department of Developmental Genetics, Chiba University Graduate School of Medicine, Chiba, Japan.
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MeSH Terms
Binding Sites / genetics
DNA / genetics,  metabolism
Escherichia coli
Homeodomain Proteins / genetics*,  metabolism
Protein Binding
Sequence Analysis, DNA
Transcriptional Activation*
Reg. No./Substance:
0/Homeodomain Proteins; 0/TLX2 protein, human; 9007-49-2/DNA

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