Document Detail

Identification, ontogeny, and regulation of an interleukin-6-like factor in the rat seminiferous tubule.
MedLine Citation:
PMID:  8380379     Owner:  NLM     Status:  MEDLINE    
The present study's aims are to search for the presence of interleukin-6 bioactivity (IL-6) in medium conditioned by various testicular cell types and to investigate the cellular and hormonal regulation of testicular IL-6 production. Sertoli cells prepared from rats of increasing ages (20, 35, and 45 days) secreted IL-6 in vitro, whereas medium conditioned by pachytene spermatocytes, early spermatids, and peritubular cells showed no activity. Lipopolysaccharide (LPS) and latex beads, two known stimulators of monocyte/macrophage IL-6 production, markedly stimulated IL-6 secretion by Sertoli cells at all the ages investigated. Maximum levels of IL-6 were reached after 6 h of culture of Sertoli cells with LPS and after 24 h with latex beads. When Sertoli cells were cocultured with pachytene spermatocytes, early spermatids, or fractions containing residual bodies and cytoplasts from elongated spermatids, only the latter significantly stimulated IL-6 levels. Maximum levels of IL-6 were attained by adding 2 x 10(6) residual bodies to Sertoli cells; a significant increase in IL-6 secretion was seen after 6 h, and maximum levels were observed after 24 h. The levels of IL-6 varied throughout different stages of the seminiferous epithelium cycle; highest levels were observed in stages II-VI and lowest in stages VII-VIII. IL-6 bioactivities induced by LPS and residual bodies and cytoplasts from elongated spermatids could be totally neutralized with a specific monoclonal antibody at all of the ages studied. FSH, phorbol myristate acetate, and IL-1 alpha augmented Sertoli cell IL-6 secretion in a dose-dependent manner. Furthermore, FSH and (Bu)2cAMP differentially stimulated IL-6 secretion during the seminiferous epithelial cycle. It is concluded that the release of IL-6 from Sertoli cells is regulated by a complex interplay between residual bodies and humoral factors.
V Syed; N Gérard; A Kaipia; C W Bardin; M Parvinen; B Jégou
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  132     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-02-05     Completed Date:  1993-02-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  293-9     Citation Subset:  AIM; IM    
GERM, INSERM CJF 91-04, Université de Rennes 1, Bretagne, France.
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MeSH Terms
Aging / metabolism
Bucladesine / pharmacology
Cells, Cultured
Follicle Stimulating Hormone / pharmacology
Interleukin-6 / metabolism*
Lipopolysaccharides / pharmacology
Rats, Sprague-Dawley
Seminiferous Epithelium / physiology
Seminiferous Tubules / growth & development*,  metabolism
Sertoli Cells / metabolism*
Spermatids / metabolism
Spermatozoa / metabolism
Grant Support
Reg. No./Substance:
0/Interleukin-6; 0/Lipopolysaccharides; 362-74-3/Bucladesine; 9002-68-0/Follicle Stimulating Hormone

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