| Identification of omega hydroxy fatty acids in biological samples as their pentafluoropropyl derivatives by gas chromatography/mass spectrometry with positive and negative ion detection. | |
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MedLine Citation:
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PMID: 12125016 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple one-step procedure for derivatization of the omega hydroxy fatty acids 20-hydroxyeicotetraeonic acid and 12-hydroxylauric acid is presented. The procedure involves acylation of the terminal hydroxy group and esterification of the carboxylic acid with a mixture of pentafluoropropionic anhydride and pentafluoropropanol. Positive and negative ion spectra for the derivatives are presented. The procedure was used to demonstrate conversion of arachidonic acid to 20-hydroxyeicosatetraeonic acid and lauric acid to 12-hydroxylauric acid in kidney microsomal incubations. The reaction appears to be specific, since derivatives of subterminal fatty acids (secondary alcohols) could not be detected. |
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Authors:
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Michael Zemaitis; Samuel Poloyac; Reginald Frye |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 16 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-07-18 Completed Date: 2002-09-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 1411-5 Citation Subset: IM |
Copyright Information:
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Copyright 2002 John Wiley & Sons, Ltd. |
Affiliation:
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University of Pittsburgh School of Pharmacy, Department of Pharmaceutical Sciences, 904 Salk Hall, Pittsburgh, PA 15261, USA. maz@pitt.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Esters / chemistry* Fatty Alcohols / chemistry Gas Chromatography-Mass Spectrometry Hydrocarbons, Fluorinated / chemistry* Hydroxyeicosatetraenoic Acids / analysis, metabolism Indicators and Reagents Kidney / chemistry, metabolism Lauric Acids / analysis Male Microsomes / chemistry, metabolism Rats Rats, Sprague-Dawley Solutions |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Fatty Alcohols; 0/Hydrocarbons, Fluorinated; 0/Hydroxyeicosatetraenoic Acids; 0/Indicators and Reagents; 0/Lauric Acids; 0/Solutions; 79551-86-3/20-hydroxy-5,8,11,14-eicosatetraenoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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