| Identification of nutrient-dependent changes in extracellular pH and acid phosphatase secretion in Aspergillus nidulans. | |
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MedLine Citation:
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PMID: 18050093 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study was designed to identify nutrient-dependent changes in extracellular pH and acid phosphatase secretion in the biA1 palC4 mutant strain of Aspergillus nidulans. The palC4 mutant was selected as lacking alkaline phosphatase, but having substantially increased acid phosphatase activity when grown on solid minimal medium under phosphate starvation, pH 6.5. Gene palC was identified as a putative member of a conserved signaling cascade involved in ambient alkaline sensing whose sole function is to promote the proteolytic activation of PacC at alkaline pH. We showed that both poor growth and conidiation of the palC4 mutant strain on solid medium, alkaline pH, were relative to its hypersensitivity to Tris (hydroxymethyl) aminomethane buffer. Also, the secretion of acid phosphatase was repressed when both the wild-type and palC4 mutant strains were grown in low-phosphate yeast extract liquid medium, pH 5.0, indicating that the secretion of this enzyme is not necessary to regenerate inorganic phosphate from the organic phosphate pool present in yeast extract. |
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Authors:
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J S Freitas; E M Silva; A Rossi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-30 |
Journal Detail:
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Title: Genetics and molecular research : GMR Volume: 6 ISSN: 1676-5680 ISO Abbreviation: Genet. Mol. Res. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-11-30 Completed Date: 2008-01-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101169387 Medline TA: Genet Mol Res Country: Brazil |
Other Details:
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Languages: eng Pagination: 721-9 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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secretion* Aspergillus nidulans / enzymology, growth & development, metabolism* Colony Count, Microbial Food Hydrogen-Ion Concentration |
| Chemical | |
Reg. No./Substance:
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EC 3.1.3.2/Acid Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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