Document Detail

Identification of nucleolus organizer regions (NORs) in normal and neoplastic human cells by the silver-staining technique.
MedLine Citation:
PMID:  74306     Owner:  NLM     Status:  MEDLINE    
Silver nitrate has been used to demonstrate the chromosomal location of ribosomal cistrons in nine tissue-culture lines derived from human tumors of various pathological origins. Control individuals have a particular modal number (range 7--10) of D- and G-group chromosomes stained with silver. In the controls, 96.2% of the D- and G-group chromosomes that have a stalk show silver staining, while no relationship can be seen in acrocentric chromosomes without stalks. The tumor cells, whose modal chromosome numbers range from 42 to 68, possess variable numbers of acrocentrics (11--18). The number of chromosomes stained with silver, however, remained at control levels (range, 6--9). These data indicate that, in humans, silver staining may not identify all NORs that contain structural ribosomal genes.
H R Hubbell; T C Hsu
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  19     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1977  
Date Detail:
Created Date:  1978-03-21     Completed Date:  1978-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  185-96     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Nucleolus / ultrastructure*
Neoplasms / genetics*,  ultrastructure
Ribosomes / ultrastructure
Silver Nitrate / diagnostic use*
Staining and Labeling
Reg. No./Substance:
7761-88-8/Silver Nitrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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