Document Detail


Identification of novel urinary metabolites of the lipid peroxidation product 4-hydroxy-2-nonenal in rats.
MedLine Citation:
PMID:  9815199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Following iv administration of 4-hydroxy-2-nonenal (HNE) and [4-3H]HNE to rats, 15 polar urinary metabolites accounting for about 50% of the urinary radioactivity were separated by HPLC. Among them, eight major compounds and tritiated water were quantified. The metabolites were unequivocally characterized using GC/MS and ESI/MS/MS/MS. Most of "HNE polar metabolites" originate from omega-oxidation of 4-hydroxy-2-nonenoic acid (HNA): 9-hydroxy-HNA, its mercapturic acid conjugate, and two diastereoisomers of the corresponding lactone. The oxidation of 9-hydroxy-HNA by alcohol and aldehyde dehydrogenases leads to the excretion of 9-carboxy-HNA and of the corresponding lactone mercapturic acid conjugate. 1, 4-Dihydroxy-2-nonene (DHN) originating from the reduction of HNE by alcohol dehydrogenase was to a lesser extent omega-hydroxylated, leading to 9-hydroxy-DHN which was excreted as a mercapturic acid conjugate (two diastereoisomers).
Authors:
J Alary; L Debrauwer; Y Fernandez; A Paris; J P Cravedi; L Dolo; D Rao; G Bories
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Chemical research in toxicology     Volume:  11     ISSN:  0893-228X     ISO Abbreviation:  Chem. Res. Toxicol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-01-11     Completed Date:  1999-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8807448     Medline TA:  Chem Res Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1368-76     Citation Subset:  IM    
Affiliation:
Laboratoire des Xénobiotiques, INRA, BP3, 31931 Toulouse Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Aldehydes / metabolism,  urine*
Animals
Biotransformation
Chromatography, High Pressure Liquid
Cysteine Proteinase Inhibitors / metabolism,  urine*
Gas Chromatography-Mass Spectrometry
Hydrogen-Ion Concentration
Lipid Peroxidation
Male
Microsomes, Liver / drug effects,  metabolism
Rats
Rats, Wistar
Spectrometry, Mass, Fast Atom Bombardment
Chemical
Reg. No./Substance:
0/Aldehydes; 0/Cysteine Proteinase Inhibitors; 29343-52-0/4-hydroxy-2-nonenal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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