| Identification of a novel staining pattern of bile duct epithelial cells in primary sclerosing cholangitis. | |
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MedLine Citation:
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PMID: 19653288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Primary sclerosing cholangitis (PSC) is an inflammatory disease of the bile ducts with an unknown etiology. A number of autoantigens have been proposed, but an early diagnostic marker is still lacking. Our aim was to identify such an autoantigen. METHODS: Immunostaining was performed on normal human bile duct with sera from patients with PSC and controls. To identify an autoantigen a cDNA library from normal human choledochus was constructed and immunoscreened with patient sera. Using in vitro transcription and translation and immunoprecipitation we examined the immunoreactivity against PDZ domain containing 1 (PDZK1) in 35 patients with PSC, 198 control patients, and 94 healthy controls. RESULTS: We observed a previously unpublished staining pattern in which cytoplasmatic granules and apical cell membranes of biliary epithelial cells were stained by PSC sera. Strong immunoreactivity to these structures was obtained with 12 out of 35 PSC sera (34%) but not with sera from healthy controls. By screening the cDNA library we identified PDZK1 as a candidate antigen. Immunoreactivity against PDZK1 was detected in 9% of PSC patients, 2% of inflammatory bowel disease (IBD) patients, 8% of autoimmune pancreatitis patients, 18% of Grave's disease patients, and 1% of healthy controls. CONCLUSIONS: Previously unpublished, specific, and strong autoantibodies against epithelial cells of the bile duct in PSC sera were identified. Furthermore, PDZK1 is suggested as a potential new autoantigen. |
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Authors:
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Brita Ardesj?; Guida M Portela-Gomes; Fredrik Rorsman; Lars Grimelius; Olov Ekwall |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Inflammatory bowel diseases Volume: 16 ISSN: 1536-4844 ISO Abbreviation: Inflamm. Bowel Dis. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-18 Completed Date: 2010-03-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508162 Medline TA: Inflamm Bowel Dis Country: United States |
Other Details:
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Languages: eng Pagination: 305-11 Citation Subset: IM |
Affiliation:
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Department of Medical Sciences University Hospital, Uppsala University, Uppsala, Sweden. brita.ardesjo@medsci.uu.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Autoantigens / diagnostic use, genetics Bile Ducts / pathology* Cholangitis, Sclerosing / pathology* Coloring Agents / diagnostic use Common Bile Duct / pathology Epithelium / pathology Fluorescent Antibody Technique Humans Middle Aged Radioimmunoprecipitation Assay Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Autoantigens; 0/Coloring Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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