Document Detail


Identification of novel serum biomarkers of hepatocellular carcinoma using glycomic analysis.
MedLine Citation:
PMID:  23322672     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The altered N-glycosylation of glycoproteins has been suggested to play an important role in the behavior of malignant cells. Using glycomics technology, we attempted to determine the specific and detailed N-glycan profile for hepatocellular carcinoma (HCC) and investigate the prognostic capabilities. From 1999 to 2011, 369 patients underwent primary curative hepatectomy in our facility and were followed up for a median of 60.7 months. As normal controls, 26 living Japanese related liver transplantation donors were selected not infected by hepatitis B and C virus. Their mean age was 40.0 and 15 (57.7%) were male. We used a glycoblotting method to purify N-glycans from preoperative blood samples from this cohort (10 μL serum) which were then identified and quantified using mass spectrometry (MS). Correlations between the N-glycan levels and the clinicopathologic characteristics and outcomes for these patients were evaluated. Our analysis of the relative areas of all the sugar peaks identified by MS, totaling 67 N-glycans, revealed that a proportion had higher relative areas in the HCC cases compared with the normal controls. Fourteen of these molecules had an area under the curve of greater than 0.80. Analysis of the correlation between these 14 N-glycans and surgical outcomes by univariate and multivariate analysis identified G2890 (m/z value, 2890.052) as a significant recurrence factor and G3560 (m/z value, 3560.295) as a significant prognostic factor. G2890 and G3560 were found to be strongly correlated with tumor number, size, and vascular invasion. Conclusion: Quantitative glycoblotting based on whole serum N-glycan profiling is an effective approach to screening for new biomarkers. The G2890 and G3560 N-glycans determined by tumor glycomics appear to be promising biomarkers for malignant behavior in HCCs. (HEPATOLOGY 2013;).
Authors:
Toshiya Kamiyama; Hideki Yokoo; Jun-Ichi Furukawa; Masaki Kurogochi; Tomoaki Togashi; Nobuaki Miura; Kazuaki Nakanishi; Hirofumi Kamachi; Tatsuhiko Kakisaka; Yosuke Tsuruga; Masato Fujiyoshi; Akinobu Taketomi; Shin-Ichiro Nishimura; Satoru Todo
Publication Detail:
Type:  Journal Article     Date:  2013-04-26
Journal Detail:
Title:  Hepatology (Baltimore, Md.)     Volume:  57     ISSN:  1527-3350     ISO Abbreviation:  Hepatology     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-13     Completed Date:  2013-08-16     Revised Date:  2014-02-10    
Medline Journal Info:
Nlm Unique ID:  8302946     Medline TA:  Hepatology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2314-25     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 American Association for the Study of Liver Diseases.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Carcinoma, Hepatocellular / blood*,  diagnosis,  mortality,  surgery
Case-Control Studies
Cause of Death
Disease-Free Survival
Female
Glycomics*
Hepatectomy
Humans
Japan / epidemiology
Liver Neoplasms / blood*,  diagnosis,  mortality,  surgery
Male
Middle Aged
Multivariate Analysis
Polysaccharides / blood*
ROC Curve
Recurrence
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tumor Markers, Biological / blood*
Young Adult
Chemical
Reg. No./Substance:
0/Polysaccharides; 0/Tumor Markers, Biological
Comments/Corrections
Comment In:
Hepatology. 2014 Jan;59(1):355-6   [PMID:  23729393 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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