| Identification of a novel protein binding to hepatitis C virus core protein. | |
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MedLine Citation:
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PMID: 19486448 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Hepatitis C virus (HCV) core protein is a multi-functional viral protein that interacts with several target proteins of both viral and cellular origin. AIM AND METHODS: To gain insight into the mechanism of action of HCV core protein, we used a yeast two-hybrid system to identify the core protein-interacting cellular targets. RESULTS: A cDNA clone encoding an aspartoacylase was obtained, termed aspartoacylase 3 (ACY3). Interaction between ACY3 and HCV core protein was verified using a co-immunoprecipitation assay in vitro, and a mammalian two-hybrid system in vivo. Fluorescence microscopy showed green fluorescence protein-fused ACY3 localized in the cytoplasm. CONCLUSION: Our data suggest that ACY3 is an HCV core binding protein, which may play a role in the development of HCV-associated diseases. |
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Authors:
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Yun-Ru Chen; Tian-Yan Chen; Shu-Lin Zhang; Shu-Mei Lin; Ying-Ren Zhao; Feng Ye; Xi Zhang; Lei Shi; Shuang-Suo Dang; Min Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-13 |
Journal Detail:
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Title: Journal of gastroenterology and hepatology Volume: 24 ISSN: 1440-1746 ISO Abbreviation: J. Gastroenterol. Hepatol. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-08-17 Completed Date: 2009-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8607909 Medline TA: J Gastroenterol Hepatol Country: Australia |
Other Details:
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Languages: eng Pagination: 1300-4 Citation Subset: IM |
Affiliation:
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The First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidohydrolases
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genetics,
metabolism* Amino Acid Sequence Animals Binding Sites COS Cells Cercopithecus aethiops Cytosol / enzymology Hepacivirus / genetics, metabolism* Humans Immunoprecipitation Liver / enzymology* Microscopy, Confocal Microscopy, Fluorescence Molecular Sequence Data Protein Interaction Domains and Motifs Protein Interaction Mapping Recombinant Fusion Proteins / metabolism Transfection Two-Hybrid System Techniques Viral Core Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Fusion Proteins; 0/Viral Core Proteins; EC 3.5.-/Amidohydrolases; EC 3.5.1.15/aspartoacylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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