Document Detail

Identification of a novel protein binding to hepatitis C virus core protein.
MedLine Citation:
PMID:  19486448     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Hepatitis C virus (HCV) core protein is a multi-functional viral protein that interacts with several target proteins of both viral and cellular origin. AIM AND METHODS: To gain insight into the mechanism of action of HCV core protein, we used a yeast two-hybrid system to identify the core protein-interacting cellular targets. RESULTS: A cDNA clone encoding an aspartoacylase was obtained, termed aspartoacylase 3 (ACY3). Interaction between ACY3 and HCV core protein was verified using a co-immunoprecipitation assay in vitro, and a mammalian two-hybrid system in vivo. Fluorescence microscopy showed green fluorescence protein-fused ACY3 localized in the cytoplasm. CONCLUSION: Our data suggest that ACY3 is an HCV core binding protein, which may play a role in the development of HCV-associated diseases.
Yun-Ru Chen; Tian-Yan Chen; Shu-Lin Zhang; Shu-Mei Lin; Ying-Ren Zhao; Feng Ye; Xi Zhang; Lei Shi; Shuang-Suo Dang; Min Liu
Related Documents :
21182168 - Protein intensity changes in the hemoglobin and plasma electrophoretic patterns of oreo...
8254778 - Sendai virus m protein binds independently to either the f or the hn glycoprotein in vivo.
2157868 - Recombinant virus vaccine for bluetongue disease in sheep.
7464948 - Structure of the protein and dna in fd filamentous bacterial virus.
18231988 - Ftir microspectroscopic analysis of the effects of certain drugs on oxidative stress an...
19230958 - Proteomic profiling in apolipoprotein e-deficient mice during atherosclerosis progression.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-13
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  24     ISSN:  1440-1746     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-08-17     Completed Date:  2009-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  1300-4     Citation Subset:  IM    
The First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amidohydrolases / genetics,  metabolism*
Amino Acid Sequence
Binding Sites
COS Cells
Cercopithecus aethiops
Cytosol / enzymology
Hepacivirus / genetics,  metabolism*
Liver / enzymology*
Microscopy, Confocal
Microscopy, Fluorescence
Molecular Sequence Data
Protein Interaction Domains and Motifs
Protein Interaction Mapping
Recombinant Fusion Proteins / metabolism
Two-Hybrid System Techniques
Viral Core Proteins / genetics,  metabolism*
Reg. No./Substance:
0/Recombinant Fusion Proteins; 0/Viral Core Proteins; EC 3.5.-/Amidohydrolases; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Predictors of microscopic portal vein invasion by hepatocellular carcinoma: measurement of portal pe...
Next Document:  Performance of acidified 14C-urea capsule breath test during pantoprazole and ranitidine treatment.