Document Detail


Identification of a novel protein binding to hepatitis C virus core protein.
MedLine Citation:
PMID:  19486448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Hepatitis C virus (HCV) core protein is a multi-functional viral protein that interacts with several target proteins of both viral and cellular origin. AIM AND METHODS: To gain insight into the mechanism of action of HCV core protein, we used a yeast two-hybrid system to identify the core protein-interacting cellular targets. RESULTS: A cDNA clone encoding an aspartoacylase was obtained, termed aspartoacylase 3 (ACY3). Interaction between ACY3 and HCV core protein was verified using a co-immunoprecipitation assay in vitro, and a mammalian two-hybrid system in vivo. Fluorescence microscopy showed green fluorescence protein-fused ACY3 localized in the cytoplasm. CONCLUSION: Our data suggest that ACY3 is an HCV core binding protein, which may play a role in the development of HCV-associated diseases.
Authors:
Yun-Ru Chen; Tian-Yan Chen; Shu-Lin Zhang; Shu-Mei Lin; Ying-Ren Zhao; Feng Ye; Xi Zhang; Lei Shi; Shuang-Suo Dang; Min Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-13
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  24     ISSN:  1440-1746     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-08-17     Completed Date:  2009-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  1300-4     Citation Subset:  IM    
Affiliation:
The First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
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MeSH Terms
Descriptor/Qualifier:
Amidohydrolases / genetics,  metabolism*
Amino Acid Sequence
Animals
Binding Sites
COS Cells
Cercopithecus aethiops
Cytosol / enzymology
Hepacivirus / genetics,  metabolism*
Humans
Immunoprecipitation
Liver / enzymology*
Microscopy, Confocal
Microscopy, Fluorescence
Molecular Sequence Data
Protein Interaction Domains and Motifs
Protein Interaction Mapping
Recombinant Fusion Proteins / metabolism
Transfection
Two-Hybrid System Techniques
Viral Core Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Recombinant Fusion Proteins; 0/Viral Core Proteins; EC 3.5.-/Amidohydrolases; EC 3.5.1.15/aspartoacylase

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