Document Detail


Identification of a novel polymorphism in the X-chromosome region homologous to the DYS456 locus.
MedLine Citation:
PMID:  16566768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During an extensive multipopulation study with Y-short tandem repeat (STR) loci, amplified using the AmpFlSTR Yfiler PCR amplification kit, amplification of a 71 bp fragment was observed in 2.32% of the male samples analyzed (N = 3141). By direct sequencing of this fragment, it was determined that the primer binding sequences were identical to those of the DYS456 locus. A T to G single-nucleotide polymorphism (SNP) enabled amplification of the 71 bp fragment. The SNP is located within an X-Y homologous region at Xq21.31 and was observed with the highest frequency within the African American and Sub-Saharan African populations in our study. Presence of SNP on the X chromosome did not interfere with the reliability of typing the DYS456 locus and the other Y-STR loci typeable using the AmpFlSTR Yfiler PCR amplification kit. Full profiles in a mixture of male:female at 1:4000 were obtained using the current configuration of the AmpFlSTR Yfiler kit even in the presence of female DNA containing the G variant.
Authors:
Chien-Wei Chang; Julio J Mulero; Bruce Budowle; Lisa M Calandro; Lori K Hennessy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of forensic sciences     Volume:  51     ISSN:  0022-1198     ISO Abbreviation:  J. Forensic Sci.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-28     Completed Date:  2006-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  344-8     Citation Subset:  IM    
Affiliation:
Applied Biosystems, Foster City, CA 94404, USA. changcw@appliedbiosystems.com
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Human, X*
Chromosomes, Human, Y
Continental Population Groups / genetics
DNA Fingerprinting*
DNA Primers
Female
Gene Frequency
Humans
Male
Mutation
Polymerase Chain Reaction
Polymorphism, Single Nucleotide*
Sequence Analysis, DNA
Tandem Repeat Sequences*
Chemical
Reg. No./Substance:
0/DNA Primers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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