Document Detail

Identification of a novel nuclear domain.
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MedLine Citation:
PMID:  1999457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For most known nuclear domains (ND), specific functions have been identified. In this report we used murine mAbs and human autoantibodies to investigate precisely circumscribed structures 0.2-0.3 micron in diameter which appear as "nuclear dots" distributed throughout the nucleoplasm. Nuclear dots are metabolically stable and resistant to nuclease digestion and salt extraction. The localization of nuclear dots is separate from kinetochores, centromeres, sites of mRNA processing and tRNA synthesis, nuclear bodies, and chromosomes. The nuclear dots, therefore, represent a novel ND. Nuclear dots break down as cells enter metaphase and reassemble at telophase. In interphase cells, nuclear dots are frequently "paired," and some are visible as "doublets" when stained with one particular antiserum. The number of dot doublets increased when quiescent cells were stimulated with serum although the total number of dots did not change substantially. One of the antigens was identified as a protein with a molecular mass of approximately 55 kD showing three charge isomers in the pI range of 7.4 to 7.7. Autoantibodies affinity purified from this nuclear dot protein (NDP-55) show nuclear dots exclusively. Nuclear dot-negative rat liver parenchymal cells became positive after chemical hepatectomy, suggesting involvement of the NDP-55 in the proliferative state of cells.
Authors:
C A Ascoli; G G Maul
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  112     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-04-08     Completed Date:  1991-04-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  785-95     Citation Subset:  IM    
Affiliation:
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
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MeSH Terms
Descriptor/Qualifier:
Amanitins / pharmacology
Animals
Autoantibodies
Cell Cycle*
Cell Line
Cell Nucleus / chemistry,  ultrastructure*
Cycloheximide / pharmacology
Fluorescent Antibody Technique
Humans
Immunoblotting
Isoelectric Focusing
Liver / chemistry,  ultrastructure
Liver Regeneration
Nuclear Proteins / analysis*,  immunology
Puromycin / pharmacology
Rats
Rats, Inbred Strains
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
AR-38907/AR/NIAMS NIH HHS; CA-10815/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Amanitins; 0/Autoantibodies; 0/Nuclear Proteins; 53-79-2/Puromycin; 66-81-9/Cycloheximide
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 3 Year: 1991
Volume: 112 Issue: 5
First Page: 785 Last Page: 795
ID: 2288866
Publisher Id: 91154311
PubMed Id: 1999457

Identification of a novel nuclear domain


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