Document Detail

Identification of a novel fibroblast growth factor, FGF-23, preferentially expressed in the ventrolateral thalamic nucleus of the brain.
MedLine Citation:
PMID:  11032749     Owner:  NLM     Status:  MEDLINE    
We isolated mouse cDNA encoding a novel FGF (251 amino acids). As this is the 23rd documented FGF, we termed it FGF-23. FGF-23 has a hydrophobic amino terminus ( approximately 24 amino acids), which is a typical signal sequence. As expected, recombinant mouse FGF-23 was efficiently secreted by High Five insect cell-infected recombinant baculovirus containing the cDNA, indicating that FGF-23 is a secreted protein. We also isolated human cDNA encoding FGF-23 (251 amino acids), which is highly identical ( approximately 72% amino acid identity) to mouse FGF-23. Of human FGF family members, FGF-23 is most similar to FGF-21 and FGF-19 ( approximately 24% and approximately 22% amino acid identities, respectively). Human FGF-23 gene was localized on the chromosome 12p13 and found to be tandem linked (within 5.5 kb) to human FGF-6 gene. The expression of FGF-23 mRNA in mouse adult tissues was examined by real-time quantitative polymerase chain reaction. FGF-23 mRNA was mainly expressed in the brain and thymus at low levels. The localization of FGF-23 mRNA in the brain was examined by in situ hybridization. FGF-23 mRNA in the brain was found to be preferentially expressed in the ventrolateral thalamic nucleus. Therefore, FGF-23 is expected a unique FGF that plays roles in the function of the ventrolateral thalamic nucleus.
T Yamashita; M Yoshioka; N Itoh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  277     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-11-06     Completed Date:  2000-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  494-8     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Yoshida-Shimoadachi, Sakyo, Kyoto, 606-8501, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB037889;  AB037973
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MeSH Terms
Amino Acid Sequence
Baculoviridae / metabolism
Blotting, Western
Brain / metabolism*
Cell Line
Chromosome Mapping
Chromosomes, Human, Pair 12
DNA, Complementary / metabolism
Electrophoresis, Polyacrylamide Gel
Fibroblast Growth Factors / biosynthesis*,  chemistry,  genetics
In Situ Hybridization
Molecular Sequence Data
RNA, Complementary / metabolism
RNA, Messenger / metabolism
Recombinant Proteins / metabolism
Sequence Homology, Amino Acid
Tissue Distribution
Ventral Thalamic Nuclei / metabolism*
Reg. No./Substance:
0/DNA, Complementary; 0/FGF19 protein, human; 0/RNA, Complementary; 0/RNA, Messenger; 0/Recombinant Proteins; 0/fibroblast growth factor 21; 62031-54-3/Fibroblast Growth Factors

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